Inhibition of cellular activation of retroviral replication by CD8+ T cells derived from non-human primates

J. D. Powell, D. P. Bednarik, T. M. Folks, T. Jehuda-Cohen, F. Villinger, K. W. Sell, A. A. Ansari

Research output: Contribution to journalArticlepeer-review

Abstract

To test the hypothesis that CD8+ T cells inhibit viral replication at the level of cellular activation, an Epstein-Barr virus (EBV)-transformed cell line (FEc1) from a simian immunodeficiency virus (SIV)-seropositive sooty mangabey monkey was transfected with a human CD4 gene and shown to be replication-competent for HIV-1, HIV-2 and SIV. Utilizing a dual-chamber culture system, it was found that inhibition of viral replication can be mediated by a soluble factor. The FEc1 cell line was transiently transfected with an LTR-driven CAT reporter gene. It was found that autologous CD8+ T cells markedly inhibited CAT activity. Furthermore, co-transfection of the FEc1 cell line with an LTR-driven tat plasmid and LTR-CAT was able to quantitatively mitigate the suppressive effect. Thus, this inhibition appears to be directed at cellular mechanisms of viral transcription. Control transfections with an LTR-driven CAT plasmid with a mutation at the NFkB binding site yielded no CAT activity, suggesting that most viral replication as measured by CAT activity is dependent, to a large extent, upon cellularly derived NFkB binding proteins.

Original languageEnglish (US)
Pages (from-to)473-481
Number of pages9
JournalClinical and Experimental Immunology
Volume91
Issue number3
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • AIDS
  • HIV
  • SIV
  • immunoregulation
  • virus suppression

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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