Inhibition of angiogenesis in human glioblastomas by chromosome 10 induction of thrombospondin-1

Stephanie C. Hsu, Olga V. Volpert, Peter A. Steck, Tom Mikkelsen, Peter J. Polverini, Sambasiva Rao, Pauline Chou, Noël P. Bouck

Research output: Contribution to journalArticle

Abstract

Glioblastoma multiforme is distinguished from its less malignant astrocytoma precursors by intense angiogenesis and frequent loss of tumor suppressor genes on chromosome 10. Here we link these traits by showing that when a wild-type chromosome 10 was returned to any of three human glioblastoma cell lines U251, U87, or LG11, they lost their ability to form tumors in nude mice and switched to an antiangiogenic phenotype, as measured by the inhibition of capillary endothelial cell migration and of corneal neovascularization. This change in angiogenesis was directly due to the increased secretion of a potent inhibitor of angiogenesis, thrombospondin-1, because: (a) neutralizing thrombospondin completely relieved the inhibition; (b) the inhibitory activity of thrombospondin was not dependent on transforming growth factor β; and (c) chromosome 10 introduction did not alter secreted inducing activity. The inducing activity was dependent on vascular endothelial cell growth factor and had an ED50 of 10 μg/ml in media conditioned by parental cells and 9-13 μg/ml in media conditioned by chromosome 10 revertants. Normal human astrocytes were also antiangiogenic due to secreted thrombospondin. The effect of chromosome 10 on thrombospondin production in vitro was reflected in patient material. Normal brain and lower grade astrocytomas known to retain chromosome 10 stained strongly for thrombospondin, but 12 of 13 glioblastomas, the majority of which lose chromosome 10, did not. These data indicate that the loss of tumor suppressors on chromosome 10 contributes to the aggressive malignancy or glioblastomas in part by releasing constraints on angiogenesis that are maintained by thrombospondin in lower grade tumors.

Original languageEnglish (US)
Pages (from-to)5684-5691
Number of pages8
JournalCancer Research
Volume56
Issue number24
StatePublished - Dec 15 1996
Externally publishedYes

Fingerprint

Thrombospondin 1
Chromosomes, Human, Pair 10
Human Chromosomes
Glioblastoma
Thrombospondins
Astrocytoma
Conditioned Culture Medium
Neoplasms
Corneal Neovascularization
Angiogenesis Inhibitors
Transforming Growth Factors
Tumor Suppressor Genes
Nude Mice
Astrocytes
Vascular Endothelial Growth Factor A
Cell Movement
Endothelial Cells
Phenotype
Cell Line
Brain

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hsu, S. C., Volpert, O. V., Steck, P. A., Mikkelsen, T., Polverini, P. J., Rao, S., ... Bouck, N. P. (1996). Inhibition of angiogenesis in human glioblastomas by chromosome 10 induction of thrombospondin-1. Cancer Research, 56(24), 5684-5691.

Inhibition of angiogenesis in human glioblastomas by chromosome 10 induction of thrombospondin-1. / Hsu, Stephanie C.; Volpert, Olga V.; Steck, Peter A.; Mikkelsen, Tom; Polverini, Peter J.; Rao, Sambasiva; Chou, Pauline; Bouck, Noël P.

In: Cancer Research, Vol. 56, No. 24, 15.12.1996, p. 5684-5691.

Research output: Contribution to journalArticle

Hsu, SC, Volpert, OV, Steck, PA, Mikkelsen, T, Polverini, PJ, Rao, S, Chou, P & Bouck, NP 1996, 'Inhibition of angiogenesis in human glioblastomas by chromosome 10 induction of thrombospondin-1', Cancer Research, vol. 56, no. 24, pp. 5684-5691.
Hsu SC, Volpert OV, Steck PA, Mikkelsen T, Polverini PJ, Rao S et al. Inhibition of angiogenesis in human glioblastomas by chromosome 10 induction of thrombospondin-1. Cancer Research. 1996 Dec 15;56(24):5684-5691.
Hsu, Stephanie C. ; Volpert, Olga V. ; Steck, Peter A. ; Mikkelsen, Tom ; Polverini, Peter J. ; Rao, Sambasiva ; Chou, Pauline ; Bouck, Noël P. / Inhibition of angiogenesis in human glioblastomas by chromosome 10 induction of thrombospondin-1. In: Cancer Research. 1996 ; Vol. 56, No. 24. pp. 5684-5691.
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