Inhibition of amiloride-sensitive sodium-channel activity in distal lung epithelial cells by nitric oxide

Jin Wen Ding, John Dickie, Hugh O'Brodovich, Yutaka Shintani, Bijan Rafii, David Hackam, Yoshinori Marunaka, Ori D. Rotstein

Research output: Contribution to journalArticle

Abstract

Distal lung epithelial cells (DLECs) play an active role in fluid clearance from the alveolus by virtue of their ability to actively transport Na+ from the alveolus to the interstitial space. The present study evaluated the ability of activated macrophages to modulate the bioelectric properties of DLECs. Low numbers of lipopolysaccharide (LPS)-treated macrophages were able to significantly reduce amiloride-sensitive short-circuit current (I(sc)) without affecting total I(sc) or monolayer resistance. This was associated with a rise in the flufenamic acid-sensitive component of the I(sc). The effect was reversed by the addition of N-monomethyl-L-arginine to the medium, implying a role for nitric oxide. We hypothesized that macrophages exerted their effect by expressing inducible nitric oxide synthase (iNOS) in DLECs. The products of LPS-treated macrophages increased the levels of iNOS protein and mRNA transcripts in DLECs as well as causing a rise in iNOS activity. Immunofluorescence microscopy of LPS-stimulated macrophage-DLEC cocultures with anti-nitrotyrosine antibodies provided evidence for the generation of peroxynitrite in macrophages but not in DLECs. These data indicate that activated macrophages in the lung may contribute to impaired resolution of acute respiratory distress syndrome and suggest a novel mechanism whereby nitric oxide might alter cell function by altering its ion-transporting phenotype.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume274
Issue number3 18-3
StatePublished - Mar 1998
Externally publishedYes

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Sodium Channels
Amiloride
Nitric Oxide
Epithelial Cells
Macrophages
Lung
Nitric Oxide Synthase Type II
Lipopolysaccharides
Flufenamic Acid
Peroxynitrous Acid
Adult Respiratory Distress Syndrome
Coculture Techniques
Fluorescence Microscopy
Arginine
Anti-Idiotypic Antibodies
Ions
Phenotype
Messenger RNA
Proteins

Keywords

  • Distal lung epithelium
  • Lung injury
  • Macrophages

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Physiology (medical)

Cite this

Inhibition of amiloride-sensitive sodium-channel activity in distal lung epithelial cells by nitric oxide. / Ding, Jin Wen; Dickie, John; O'Brodovich, Hugh; Shintani, Yutaka; Rafii, Bijan; Hackam, David; Marunaka, Yoshinori; Rotstein, Ori D.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 274, No. 3 18-3, 03.1998.

Research output: Contribution to journalArticle

Ding, Jin Wen ; Dickie, John ; O'Brodovich, Hugh ; Shintani, Yutaka ; Rafii, Bijan ; Hackam, David ; Marunaka, Yoshinori ; Rotstein, Ori D. / Inhibition of amiloride-sensitive sodium-channel activity in distal lung epithelial cells by nitric oxide. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1998 ; Vol. 274, No. 3 18-3.
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