Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin

Ashish Gupta, Mayank Dwivedi, Abbas Ali Mahdi, G. A Nagana Gowda, Chunni Lal Khetrapal, Mahendra Bhandari

Research output: Contribution to journalArticle

Abstract

Proanthocyanidin is commonly used for inhibiting urinary tract infection (UTI) of sensitive strains of Escherichia coli. The aim of this study was to investigate the effect of proanthocyanidin on adherence of uropathogenic multi-drug resistant E. coli to uroepithelial cells, which has not yet been investigated so far. Extracts of the purified proanthocyanidin were prepared from dried cranberry juice. Purity and structural assignment of proanthocyanidin was assessed using high performance liquid chromatography and 13C nuclear magnetic resonance spectroscopy, respectively. Subsequently, its affect on multi-drug resistant bacteria as well as quantification of anti-adherence bioactivity on human vaginal and bladder epithelial cells was appraised. Inhibition of adherence to an extent of about 70% with multi-drug resistant E. coli strains was observed on uroepithelial cell. The anti-adherence bioactivity of the proanthocyanidin was detected at concentrations of 10-50 lg/ml with significant bacteriuria. Probable proanthocyanidin through A-type linkages either combines to P-fimbriae of bacterial cells or modifies the structural entity of P-fimbriae and inhibits bacterial adherence to uroepithelial cells. The proanthocyanidin exhibited anti-adherence property with multi-drug resistant strains of uropathogenic P-fimbriated E. coli with in vitro study. Hence proanthocyanidin may be considered as an inhibitory agent for multi-drug resistant strains of E. coli adherence to uroepithelial cells.

Original languageEnglish (US)
Pages (from-to)143-150
Number of pages8
JournalUrological Research
Volume40
Issue number2
DOIs
StatePublished - Apr 2012
Externally publishedYes

Fingerprint

Escherichia coli
Pharmaceutical Preparations
Bacterial Fimbriae
Vaccinium macrocarpon
Bacteriuria
Inhibition (Psychology)
proanthocyanidin
Urinary Tract Infections
Urinary Bladder
Magnetic Resonance Spectroscopy
Epithelial Cells
High Pressure Liquid Chromatography
Bacteria

Keywords

  • Bacterial adherence
  • Multi-drug resistance E. coli
  • Proanthocyanidin

ASJC Scopus subject areas

  • Urology

Cite this

Gupta, A., Dwivedi, M., Mahdi, A. A., Gowda, G. A. N., Khetrapal, C. L., & Bhandari, M. (2012). Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin. Urological Research, 40(2), 143-150. https://doi.org/10.1007/s00240-011-0398-2

Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin. / Gupta, Ashish; Dwivedi, Mayank; Mahdi, Abbas Ali; Gowda, G. A Nagana; Khetrapal, Chunni Lal; Bhandari, Mahendra.

In: Urological Research, Vol. 40, No. 2, 04.2012, p. 143-150.

Research output: Contribution to journalArticle

Gupta, A, Dwivedi, M, Mahdi, AA, Gowda, GAN, Khetrapal, CL & Bhandari, M 2012, 'Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin', Urological Research, vol. 40, no. 2, pp. 143-150. https://doi.org/10.1007/s00240-011-0398-2
Gupta A, Dwivedi M, Mahdi AA, Gowda GAN, Khetrapal CL, Bhandari M. Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin. Urological Research. 2012 Apr;40(2):143-150. https://doi.org/10.1007/s00240-011-0398-2
Gupta, Ashish ; Dwivedi, Mayank ; Mahdi, Abbas Ali ; Gowda, G. A Nagana ; Khetrapal, Chunni Lal ; Bhandari, Mahendra. / Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin. In: Urological Research. 2012 ; Vol. 40, No. 2. pp. 143-150.
@article{8cfc8e8f4d7e405bb382ac53a710c150,
title = "Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin",
abstract = "Proanthocyanidin is commonly used for inhibiting urinary tract infection (UTI) of sensitive strains of Escherichia coli. The aim of this study was to investigate the effect of proanthocyanidin on adherence of uropathogenic multi-drug resistant E. coli to uroepithelial cells, which has not yet been investigated so far. Extracts of the purified proanthocyanidin were prepared from dried cranberry juice. Purity and structural assignment of proanthocyanidin was assessed using high performance liquid chromatography and 13C nuclear magnetic resonance spectroscopy, respectively. Subsequently, its affect on multi-drug resistant bacteria as well as quantification of anti-adherence bioactivity on human vaginal and bladder epithelial cells was appraised. Inhibition of adherence to an extent of about 70{\%} with multi-drug resistant E. coli strains was observed on uroepithelial cell. The anti-adherence bioactivity of the proanthocyanidin was detected at concentrations of 10-50 lg/ml with significant bacteriuria. Probable proanthocyanidin through A-type linkages either combines to P-fimbriae of bacterial cells or modifies the structural entity of P-fimbriae and inhibits bacterial adherence to uroepithelial cells. The proanthocyanidin exhibited anti-adherence property with multi-drug resistant strains of uropathogenic P-fimbriated E. coli with in vitro study. Hence proanthocyanidin may be considered as an inhibitory agent for multi-drug resistant strains of E. coli adherence to uroepithelial cells.",
keywords = "Bacterial adherence, Multi-drug resistance E. coli, Proanthocyanidin",
author = "Ashish Gupta and Mayank Dwivedi and Mahdi, {Abbas Ali} and Gowda, {G. A Nagana} and Khetrapal, {Chunni Lal} and Mahendra Bhandari",
year = "2012",
month = "4",
doi = "10.1007/s00240-011-0398-2",
language = "English (US)",
volume = "40",
pages = "143--150",
journal = "Urolithiasis",
issn = "2194-7228",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Inhibition of adherence of multi-drug resistant E. coli by proanthocyanidin

AU - Gupta, Ashish

AU - Dwivedi, Mayank

AU - Mahdi, Abbas Ali

AU - Gowda, G. A Nagana

AU - Khetrapal, Chunni Lal

AU - Bhandari, Mahendra

PY - 2012/4

Y1 - 2012/4

N2 - Proanthocyanidin is commonly used for inhibiting urinary tract infection (UTI) of sensitive strains of Escherichia coli. The aim of this study was to investigate the effect of proanthocyanidin on adherence of uropathogenic multi-drug resistant E. coli to uroepithelial cells, which has not yet been investigated so far. Extracts of the purified proanthocyanidin were prepared from dried cranberry juice. Purity and structural assignment of proanthocyanidin was assessed using high performance liquid chromatography and 13C nuclear magnetic resonance spectroscopy, respectively. Subsequently, its affect on multi-drug resistant bacteria as well as quantification of anti-adherence bioactivity on human vaginal and bladder epithelial cells was appraised. Inhibition of adherence to an extent of about 70% with multi-drug resistant E. coli strains was observed on uroepithelial cell. The anti-adherence bioactivity of the proanthocyanidin was detected at concentrations of 10-50 lg/ml with significant bacteriuria. Probable proanthocyanidin through A-type linkages either combines to P-fimbriae of bacterial cells or modifies the structural entity of P-fimbriae and inhibits bacterial adherence to uroepithelial cells. The proanthocyanidin exhibited anti-adherence property with multi-drug resistant strains of uropathogenic P-fimbriated E. coli with in vitro study. Hence proanthocyanidin may be considered as an inhibitory agent for multi-drug resistant strains of E. coli adherence to uroepithelial cells.

AB - Proanthocyanidin is commonly used for inhibiting urinary tract infection (UTI) of sensitive strains of Escherichia coli. The aim of this study was to investigate the effect of proanthocyanidin on adherence of uropathogenic multi-drug resistant E. coli to uroepithelial cells, which has not yet been investigated so far. Extracts of the purified proanthocyanidin were prepared from dried cranberry juice. Purity and structural assignment of proanthocyanidin was assessed using high performance liquid chromatography and 13C nuclear magnetic resonance spectroscopy, respectively. Subsequently, its affect on multi-drug resistant bacteria as well as quantification of anti-adherence bioactivity on human vaginal and bladder epithelial cells was appraised. Inhibition of adherence to an extent of about 70% with multi-drug resistant E. coli strains was observed on uroepithelial cell. The anti-adherence bioactivity of the proanthocyanidin was detected at concentrations of 10-50 lg/ml with significant bacteriuria. Probable proanthocyanidin through A-type linkages either combines to P-fimbriae of bacterial cells or modifies the structural entity of P-fimbriae and inhibits bacterial adherence to uroepithelial cells. The proanthocyanidin exhibited anti-adherence property with multi-drug resistant strains of uropathogenic P-fimbriated E. coli with in vitro study. Hence proanthocyanidin may be considered as an inhibitory agent for multi-drug resistant strains of E. coli adherence to uroepithelial cells.

KW - Bacterial adherence

KW - Multi-drug resistance E. coli

KW - Proanthocyanidin

UR - http://www.scopus.com/inward/record.url?scp=84861418932&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861418932&partnerID=8YFLogxK

U2 - 10.1007/s00240-011-0398-2

DO - 10.1007/s00240-011-0398-2

M3 - Article

VL - 40

SP - 143

EP - 150

JO - Urolithiasis

JF - Urolithiasis

SN - 2194-7228

IS - 2

ER -