Inhibition of accelerated graft arteriosclerosis by gene transfer of soluble fibroblast growth factor receptor-1 in rat aortic transplants

Wensheng Luo, Ailian Liu, Yong Chen, Hyung M. Lim, Jennifer Marshall-Neff, James Hamilton Black, William Baldwin, Ralph H Hruban, Susan C. Stevenson, Peter Mouton, Alan Dardik, Barbara J. Ballermann

Research output: Contribution to journalArticle

Abstract

Objective-Because increased fibroblast growth factor-1 (FGF-1) and FGF receptor (FGFR) expression correlate with the development of accelerated graft arteriosclerosis in transplanted human hearts, this study sought to determine whether local gene transfer of soluble FGFR-1, capable of binding both FGF-1 and FGF-2, could blunt the development of accelerated graft arteriosclerosis in the rat aortic transplant model. Methods and Results-A construct encoding the FGFR-1 ectodomain, capable of neutralizing FGF-2 action, was expressed in rat aortic allografts, using adenoviral gene transfer at the time of transplantation. Neointima formation was inhibited in aortic allografts transduced with soluble FGFR-1, compared with allografts transduced with Null virus. Conclusion-FGFs play a causal role in the development of accelerated graft arteriosclerosis in the rat aortic transplant model. Targeted interruption of FGF function could potentially reduce neointima formation in patients with heart and kidney transplants.

Original languageEnglish (US)
Pages (from-to)1081-1086
Number of pages6
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume24
Issue number6
DOIs
StatePublished - Jun 2004

Fingerprint

Receptor, Fibroblast Growth Factor, Type 1
Arteriosclerosis
Fibroblast Growth Factor 1
Fibroblast Growth Factor Receptors
Transplants
Genes
Allografts
Neointima
Fibroblast Growth Factor 2
Transplantation
Viruses
Kidney

Keywords

  • Chronic rejection
  • Neointima
  • Transplant vasculopathy
  • Transplantation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Inhibition of accelerated graft arteriosclerosis by gene transfer of soluble fibroblast growth factor receptor-1 in rat aortic transplants. / Luo, Wensheng; Liu, Ailian; Chen, Yong; Lim, Hyung M.; Marshall-Neff, Jennifer; Black, James Hamilton; Baldwin, William; Hruban, Ralph H; Stevenson, Susan C.; Mouton, Peter; Dardik, Alan; Ballermann, Barbara J.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 24, No. 6, 06.2004, p. 1081-1086.

Research output: Contribution to journalArticle

Luo, Wensheng ; Liu, Ailian ; Chen, Yong ; Lim, Hyung M. ; Marshall-Neff, Jennifer ; Black, James Hamilton ; Baldwin, William ; Hruban, Ralph H ; Stevenson, Susan C. ; Mouton, Peter ; Dardik, Alan ; Ballermann, Barbara J. / Inhibition of accelerated graft arteriosclerosis by gene transfer of soluble fibroblast growth factor receptor-1 in rat aortic transplants. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 2004 ; Vol. 24, No. 6. pp. 1081-1086.
@article{caae99a8d61144efa0297e1fc0cbe7c3,
title = "Inhibition of accelerated graft arteriosclerosis by gene transfer of soluble fibroblast growth factor receptor-1 in rat aortic transplants",
abstract = "Objective-Because increased fibroblast growth factor-1 (FGF-1) and FGF receptor (FGFR) expression correlate with the development of accelerated graft arteriosclerosis in transplanted human hearts, this study sought to determine whether local gene transfer of soluble FGFR-1, capable of binding both FGF-1 and FGF-2, could blunt the development of accelerated graft arteriosclerosis in the rat aortic transplant model. Methods and Results-A construct encoding the FGFR-1 ectodomain, capable of neutralizing FGF-2 action, was expressed in rat aortic allografts, using adenoviral gene transfer at the time of transplantation. Neointima formation was inhibited in aortic allografts transduced with soluble FGFR-1, compared with allografts transduced with Null virus. Conclusion-FGFs play a causal role in the development of accelerated graft arteriosclerosis in the rat aortic transplant model. Targeted interruption of FGF function could potentially reduce neointima formation in patients with heart and kidney transplants.",
keywords = "Chronic rejection, Neointima, Transplant vasculopathy, Transplantation",
author = "Wensheng Luo and Ailian Liu and Yong Chen and Lim, {Hyung M.} and Jennifer Marshall-Neff and Black, {James Hamilton} and William Baldwin and Hruban, {Ralph H} and Stevenson, {Susan C.} and Peter Mouton and Alan Dardik and Ballermann, {Barbara J.}",
year = "2004",
month = "6",
doi = "10.1161/01.ATV.0000128201.65443.ea",
language = "English (US)",
volume = "24",
pages = "1081--1086",
journal = "Arteriosclerosis, Thrombosis, and Vascular Biology",
issn = "1079-5642",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Inhibition of accelerated graft arteriosclerosis by gene transfer of soluble fibroblast growth factor receptor-1 in rat aortic transplants

AU - Luo, Wensheng

AU - Liu, Ailian

AU - Chen, Yong

AU - Lim, Hyung M.

AU - Marshall-Neff, Jennifer

AU - Black, James Hamilton

AU - Baldwin, William

AU - Hruban, Ralph H

AU - Stevenson, Susan C.

AU - Mouton, Peter

AU - Dardik, Alan

AU - Ballermann, Barbara J.

PY - 2004/6

Y1 - 2004/6

N2 - Objective-Because increased fibroblast growth factor-1 (FGF-1) and FGF receptor (FGFR) expression correlate with the development of accelerated graft arteriosclerosis in transplanted human hearts, this study sought to determine whether local gene transfer of soluble FGFR-1, capable of binding both FGF-1 and FGF-2, could blunt the development of accelerated graft arteriosclerosis in the rat aortic transplant model. Methods and Results-A construct encoding the FGFR-1 ectodomain, capable of neutralizing FGF-2 action, was expressed in rat aortic allografts, using adenoviral gene transfer at the time of transplantation. Neointima formation was inhibited in aortic allografts transduced with soluble FGFR-1, compared with allografts transduced with Null virus. Conclusion-FGFs play a causal role in the development of accelerated graft arteriosclerosis in the rat aortic transplant model. Targeted interruption of FGF function could potentially reduce neointima formation in patients with heart and kidney transplants.

AB - Objective-Because increased fibroblast growth factor-1 (FGF-1) and FGF receptor (FGFR) expression correlate with the development of accelerated graft arteriosclerosis in transplanted human hearts, this study sought to determine whether local gene transfer of soluble FGFR-1, capable of binding both FGF-1 and FGF-2, could blunt the development of accelerated graft arteriosclerosis in the rat aortic transplant model. Methods and Results-A construct encoding the FGFR-1 ectodomain, capable of neutralizing FGF-2 action, was expressed in rat aortic allografts, using adenoviral gene transfer at the time of transplantation. Neointima formation was inhibited in aortic allografts transduced with soluble FGFR-1, compared with allografts transduced with Null virus. Conclusion-FGFs play a causal role in the development of accelerated graft arteriosclerosis in the rat aortic transplant model. Targeted interruption of FGF function could potentially reduce neointima formation in patients with heart and kidney transplants.

KW - Chronic rejection

KW - Neointima

KW - Transplant vasculopathy

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=2942511346&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942511346&partnerID=8YFLogxK

U2 - 10.1161/01.ATV.0000128201.65443.ea

DO - 10.1161/01.ATV.0000128201.65443.ea

M3 - Article

C2 - 15072997

AN - SCOPUS:2942511346

VL - 24

SP - 1081

EP - 1086

JO - Arteriosclerosis, Thrombosis, and Vascular Biology

JF - Arteriosclerosis, Thrombosis, and Vascular Biology

SN - 1079-5642

IS - 6

ER -