Inhaled 45-50% argon augments hypothermic brain protection in a piglet model of perinatal asphyxia

Kevin D. Broad, Igor Fierens, Bobbi Fleiss, Eridan Rocha-Ferreira, Mojgan Ezzati, Jane Hassell, Daniel Alonso-Alconada, Alan Bainbridge, Go Kawano, Daqing Ma, Ilias Tachtsidis, Pierre Gressens, Xavier Golay, Robert D. Sanders, Nicola J. Robertson

Research output: Contribution to journalArticle

Abstract

Cooling to 33.5°C in babies with neonatal encephalopathy significantly reduces death and disability, however additional therapies are needed to maximize brain protection. Following hypoxia-ischemia we assessed whether inhaled 45-50% Argon from 2-26h augmented hypothermia neuroprotection in a neonatal piglet model, using MRS and aEEG, which predict outcome in babies with neonatal encephalopathy, and immunohistochemistry. Following cerebral hypoxia-ischemia, 20 Newborn male Large White piglets1H MRS lactate/N acetyl aspartate in white (p=0.03 and 0.04) but not gray matter at 24 and 48h. EEG background recovery was faster (p2 were 23.9 points lower (95% C.I. 7.3-40.5) for the Cooling+Argon versus Cooling. Inhaled 45-50% Argon from 2-26h augmented hypothermic protection at 48h after hypoxia-ischemia shown by improved brain energy metabolism on MRS, faster EEG recovery and reduced cell death on TUNEL. Argon may provide a cheap and practical therapy to augment cooling for neonatal encephalopathy.

Original languageEnglish (US)
Pages (from-to)29-38
Number of pages10
JournalNeurobiology of Disease
Volume87
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

Fingerprint

Argon
Asphyxia
Brain Diseases
Brain
Electroencephalography
Ischemia
Brain Hypoxia-Ischemia
In Situ Nick-End Labeling
Hypothermia
Energy Metabolism
Lactic Acid
Cell Death
Immunohistochemistry
Therapeutics
Hypoxia

Keywords

  • Argon
  • Hypoxia-ischemia
  • Neonatal encephalopathy
  • Noble gas
  • Therapeutic hypothermia

ASJC Scopus subject areas

  • Neurology

Cite this

Broad, K. D., Fierens, I., Fleiss, B., Rocha-Ferreira, E., Ezzati, M., Hassell, J., ... Robertson, N. J. (2016). Inhaled 45-50% argon augments hypothermic brain protection in a piglet model of perinatal asphyxia. Neurobiology of Disease, 87, 29-38. https://doi.org/10.1016/j.nbd.2015.12.001

Inhaled 45-50% argon augments hypothermic brain protection in a piglet model of perinatal asphyxia. / Broad, Kevin D.; Fierens, Igor; Fleiss, Bobbi; Rocha-Ferreira, Eridan; Ezzati, Mojgan; Hassell, Jane; Alonso-Alconada, Daniel; Bainbridge, Alan; Kawano, Go; Ma, Daqing; Tachtsidis, Ilias; Gressens, Pierre; Golay, Xavier; Sanders, Robert D.; Robertson, Nicola J.

In: Neurobiology of Disease, Vol. 87, 01.03.2016, p. 29-38.

Research output: Contribution to journalArticle

Broad, KD, Fierens, I, Fleiss, B, Rocha-Ferreira, E, Ezzati, M, Hassell, J, Alonso-Alconada, D, Bainbridge, A, Kawano, G, Ma, D, Tachtsidis, I, Gressens, P, Golay, X, Sanders, RD & Robertson, NJ 2016, 'Inhaled 45-50% argon augments hypothermic brain protection in a piglet model of perinatal asphyxia', Neurobiology of Disease, vol. 87, pp. 29-38. https://doi.org/10.1016/j.nbd.2015.12.001
Broad, Kevin D. ; Fierens, Igor ; Fleiss, Bobbi ; Rocha-Ferreira, Eridan ; Ezzati, Mojgan ; Hassell, Jane ; Alonso-Alconada, Daniel ; Bainbridge, Alan ; Kawano, Go ; Ma, Daqing ; Tachtsidis, Ilias ; Gressens, Pierre ; Golay, Xavier ; Sanders, Robert D. ; Robertson, Nicola J. / Inhaled 45-50% argon augments hypothermic brain protection in a piglet model of perinatal asphyxia. In: Neurobiology of Disease. 2016 ; Vol. 87. pp. 29-38.
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