Infusion of hemolyzed red blood cells within peripheral blood stem cell grafts in patients with and without sickle cell disease

Courtney D. Fitzhugh, Hayato Unno, Vincent Hathaway, Wynona A. Coles, Mary E. Link, R. Patrick Weitzel, Xiongce Zhao, Elizabeth C. Wright, David F. Stroncek, Gregory J. Kato, Matthew M. Hsieh, John F. Tisdale

Research output: Contribution to journalArticle

Abstract

Peripheral blood stem cell (PBSC) infusions are associated with complications such as elevated blood pressure and decreased creatinine clearance. Patients with sickle cell disease experience similar manifestations, and some have postulated release of plasma-free hemoglobin with subsequent nitric oxide consumption as causative. We sought to evaluate whether the infusion of PBSC grafts containing lysed red blood cells (RBCs) leads to the toxicity observed in transplant subjects. We report a prospective cohort study of 60 subjects divided into 4 groups based on whether their infusions contained dimethyl sulfoxide (DMSO) and lysed RBCs, no DMSO and fresh RBCs, DMSO and no RBCs, or saline. Our primary end point, change in maximum blood pressure compared with baseline, was not significantly different among groups. Tricuspid regurgitant velocity and creatinine levels also did not differ significantly among groups. Our data do not support free hemoglobin as a significant contributor to toxicity associated with PBSC infusions. This study was registered at clinicaltrials.gov. (NCT00631787).

Original languageEnglish (US)
Pages (from-to)5671-5673
Number of pages3
JournalBlood
Volume119
Issue number24
DOIs
StatePublished - Jun 14 2012

ASJC Scopus subject areas

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

Fingerprint Dive into the research topics of 'Infusion of hemolyzed red blood cells within peripheral blood stem cell grafts in patients with and without sickle cell disease'. Together they form a unique fingerprint.

  • Cite this

    Fitzhugh, C. D., Unno, H., Hathaway, V., Coles, W. A., Link, M. E., Weitzel, R. P., Zhao, X., Wright, E. C., Stroncek, D. F., Kato, G. J., Hsieh, M. M., & Tisdale, J. F. (2012). Infusion of hemolyzed red blood cells within peripheral blood stem cell grafts in patients with and without sickle cell disease. Blood, 119(24), 5671-5673. https://doi.org/10.1182/blood-2011-11-392654