Infrequent Inactivation of the Retinoblastoma Gene Despite Frequent Loss of Chromosome 13q in Head and Neck Squamous Cell Carcinoma

George H. Yoo, Hong Ji Xu, Joseph A. Brennan, William Westra, Ralph H. Hruban, Wayne Koch, William F. Benedict, David Sidransky

Research output: Contribution to journalArticle

Abstract

Our recent allelic analysis of head and neck squamous cell carcinomas identified a high incidence of chromosomal loss on 13q. To further define an area of minimal loss, we tested 60 primary head and neck squamous cell carcinomas in 59 patients for loss of heterozygosity (LOH) by using 10 polymorphic microsatellite markers spanning the long arm of chromosome 13. We examined the same primary tumors for inactivation of the retinoblastoma (Rb) gene by immunohistochemical analysis of paraffin-embedded specimens. Thirty-one of 60 (52%) tumors demonstrated LOH in at least one 13q marker. Twenty-nine of 31 (94%) lost a portion of 13q that included D13s133, which lies just telomeric to the Rb gene at 13q14.3. However, immunohistochemical staining revealed absence of Rb protein in only 6 of these 31 tumors (19.4%) with LOH. All but one tumor without LOH on 13q displayed normal Rb protein staining. Although Rb may be inactivated by an unusual mechanism in some head and neck squamous cell carcinomas, our data suggest that another tumor suppressor gene locus at 13ql4 is likely to be involved in head and neck tumor progression.

Original languageEnglish (US)
Pages (from-to)4603-4606
Number of pages4
JournalCancer Research
Volume54
Issue number17
StatePublished - Sep 1994

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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