Infrared imaging of nitric oxide-mediated blood flow in human sickle cell disease

Alexander M. Gorbach, Hans C. Ackerman, Wei Min Liu, Joseph M. Meyer, Patricia L. Littel, Catherine Seamon, Eleni Footman, Amy Chi, Suzana Zorca, Megan L. Krajewski, Michael J. Cuttica, Roberto F. Machado, Richard O. Cannon, Gregory J. Kato

Research output: Contribution to journalArticle

Abstract

Vascular dysfunction is an important pathophysiologic manifestation of sickle cell disease (SCD), a condition that increases risk of pulmonary hypertension and stroke. We hypothesized that infrared (IR) imaging would detect changes in cutaneous bloodflow reflective of vascular function. We performed IR imaging and conventional strain gauge plethysmography in twenty-five adults with SCD at baseline and during intra-arterial infusions of an endothelium-dependent vasodilator acetylcholine (ACh), an endothelium-independent vasodilator sodium nitroprusside (SNP), and a NOS inhibitor L-NMMA. Skin temperature measured by IR imaging increased in a dose-dependent manner to graded infusions of ACh (+1.1°C, ps=0.57, p=0.003; SNP: +8.6mL/min/100mL, p2=0.84, p

Original languageEnglish (US)
Pages (from-to)262-269
Number of pages8
JournalMicrovascular Research
Volume84
Issue number3
DOIs
StatePublished - Nov 2012
Externally publishedYes

Fingerprint

Infrared imaging
Sickle Cell Anemia
Nitroprusside
Acetylcholine
Blood Vessels
Nitric Oxide
Blood
omega-N-Methylarginine
Endothelium-Dependent Relaxing Factors
Intra Arterial Infusions
Plethysmography
Skin Temperature
Vasodilator Agents
Pulmonary Hypertension
Endothelium
Stroke
Strain gages
Skin
Temperature

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Gorbach, A. M., Ackerman, H. C., Liu, W. M., Meyer, J. M., Littel, P. L., Seamon, C., ... Kato, G. J. (2012). Infrared imaging of nitric oxide-mediated blood flow in human sickle cell disease. Microvascular Research, 84(3), 262-269. https://doi.org/10.1016/j.mvr.2012.06.011

Infrared imaging of nitric oxide-mediated blood flow in human sickle cell disease. / Gorbach, Alexander M.; Ackerman, Hans C.; Liu, Wei Min; Meyer, Joseph M.; Littel, Patricia L.; Seamon, Catherine; Footman, Eleni; Chi, Amy; Zorca, Suzana; Krajewski, Megan L.; Cuttica, Michael J.; Machado, Roberto F.; Cannon, Richard O.; Kato, Gregory J.

In: Microvascular Research, Vol. 84, No. 3, 11.2012, p. 262-269.

Research output: Contribution to journalArticle

Gorbach, AM, Ackerman, HC, Liu, WM, Meyer, JM, Littel, PL, Seamon, C, Footman, E, Chi, A, Zorca, S, Krajewski, ML, Cuttica, MJ, Machado, RF, Cannon, RO & Kato, GJ 2012, 'Infrared imaging of nitric oxide-mediated blood flow in human sickle cell disease', Microvascular Research, vol. 84, no. 3, pp. 262-269. https://doi.org/10.1016/j.mvr.2012.06.011
Gorbach AM, Ackerman HC, Liu WM, Meyer JM, Littel PL, Seamon C et al. Infrared imaging of nitric oxide-mediated blood flow in human sickle cell disease. Microvascular Research. 2012 Nov;84(3):262-269. https://doi.org/10.1016/j.mvr.2012.06.011
Gorbach, Alexander M. ; Ackerman, Hans C. ; Liu, Wei Min ; Meyer, Joseph M. ; Littel, Patricia L. ; Seamon, Catherine ; Footman, Eleni ; Chi, Amy ; Zorca, Suzana ; Krajewski, Megan L. ; Cuttica, Michael J. ; Machado, Roberto F. ; Cannon, Richard O. ; Kato, Gregory J. / Infrared imaging of nitric oxide-mediated blood flow in human sickle cell disease. In: Microvascular Research. 2012 ; Vol. 84, No. 3. pp. 262-269.
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