Influences of geographic origin and cryopreservation of mononuclear cells on number of immunoglobulin secreting cells detected in human peripheral circulation

S. Baqar, A. Nour El Din, P. A. Scott, A. L. Bourgeois, M. T. Kleinoskv, A. S. Mourad, J. R. Murphy

Research output: Contribution to journalArticle

Abstract

Antibody-secreting cells (ASC) were measured in paired fresh and cryopreserved mononuclear cells (MNC) from healthy adult residents of the United States (US) or Egypt (EG). Fresh cells from US volunteers had mean ASC/10 MNC of 617,7,738 and 868 for IgM, IgG and IgA, respectively; whereas EG volunteers had 2,086,7,580 and 1,677. The US volunteers had significantly less IgM and IgA ASC (p<0.001). Cryopreservation resulted in a slight reduction in group mean isotype specific ASC (maximum reduction 14%, not statistically significant). To determine if Cryopreservation affected the number of antigen specific ASC, a group of US adults received a single oral dose of a mutated E. colt heat-labile enterotoxin (LTRi92o). Blood was collected 7 days after vaccination and ASC to LT were determined using both fresh and cryopreserved samples. Mean ASC/106 MNC were 93 vs 106 and 83 vs 86 for fresh or frozen specimens for IgG or IgA, respectively; within isotype results did not differ significantly. Demonstration that cryopreserved MNC are suitable for measurement of total as well as antigen specific ASC allows extension of this methodology to populations not previously open to evaluation and direct comparison of samples obtained at different times and places in the same assay.

Original languageEnglish (US)
Pages (from-to)A1363
JournalFASEB Journal
Volume10
Issue number6
StatePublished - Dec 1 1996
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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