Influence of thyroid hormone status on expression of genes encoding G protein subunits in the rat heart

Michael A. Levine, Arthur M. Feldman, Janet D. Robishaw, Paul W Ladenson, Thomas G. Ahn, John F. Moroney, Philip M. Smallwood

Research output: Contribution to journalArticle

Abstract

We have studied the influence of thyroid hormone status in vivo on expression of the genes encoding guanine nucleotide-binding regulatory protein (G protein) α-subunits G8α, Giα(2), Giα(3), and both the 36-kDa form (β1) and the 35-kDa form (β2) of the β-subunit in rat ventricle. The relative amounts of immunoactive Giα(2) and Giα(3) were greater in ventricular membranes from hypothyroid animals than from euthyroid animals (1.9- and 2.6-fold, respectively). A corresponding 2.3-fold increase in Giα(2) mRNA was ob-served as well as a 1.5-fold increase in Giα(3) mRNA. The relative amounts of immunoactive β1 and β2 polypeptides were also increased (2.8- and 1.8-fold, respectively) in the hypothyroid state and corresponded with comparable increases in the relative levels of β1 and β2 mRNAs. No difference was seen between the amounts of Giα(2), Giα(3), β1, and β2 in the euthyroid state and the hyperthyroid state. In contrast to these effects of thyroid hormone status on Giα and β, the steady-state amounts of Giα protein and mRNA were not altered by thyroid hormone status. Thyroid hormone status did not alter sensitivity of adenylyl cyclase to stimulation by sodium fluoride or guanyl-5′-yl imidodiphosphate (GppNHp), nor did it influence GppNHp-induced inhibition of forskolin-stimulated enzyme activity. These results demonstrate that thyroid hormone status in vivo can regulate expression of specific G protein subunits in rat myocardium. However, the physiological consequences of these changes remain unclear.

Original languageEnglish (US)
Pages (from-to)3553-3560
Number of pages8
JournalJournal of Biological Chemistry
Volume265
Issue number6
StatePublished - Feb 25 1990

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Gene encoding
Protein Subunits
Thyroid Hormones
GTP-Binding Proteins
Rats
Gene Expression
Messenger RNA
Animals
Gi-Go GTP-Binding Protein alpha Subunits
Guanylyl Imidodiphosphate
Enzyme inhibition
Sodium Fluoride
Guanine Nucleotides
Enzyme activity
Colforsin
Hyperthyroidism
Adenylyl Cyclases
Myocardium
Carrier Proteins
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Levine, M. A., Feldman, A. M., Robishaw, J. D., Ladenson, P. W., Ahn, T. G., Moroney, J. F., & Smallwood, P. M. (1990). Influence of thyroid hormone status on expression of genes encoding G protein subunits in the rat heart. Journal of Biological Chemistry, 265(6), 3553-3560.

Influence of thyroid hormone status on expression of genes encoding G protein subunits in the rat heart. / Levine, Michael A.; Feldman, Arthur M.; Robishaw, Janet D.; Ladenson, Paul W; Ahn, Thomas G.; Moroney, John F.; Smallwood, Philip M.

In: Journal of Biological Chemistry, Vol. 265, No. 6, 25.02.1990, p. 3553-3560.

Research output: Contribution to journalArticle

Levine, MA, Feldman, AM, Robishaw, JD, Ladenson, PW, Ahn, TG, Moroney, JF & Smallwood, PM 1990, 'Influence of thyroid hormone status on expression of genes encoding G protein subunits in the rat heart', Journal of Biological Chemistry, vol. 265, no. 6, pp. 3553-3560.
Levine, Michael A. ; Feldman, Arthur M. ; Robishaw, Janet D. ; Ladenson, Paul W ; Ahn, Thomas G. ; Moroney, John F. ; Smallwood, Philip M. / Influence of thyroid hormone status on expression of genes encoding G protein subunits in the rat heart. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 6. pp. 3553-3560.
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