TY - JOUR
T1 - Influence of thyroid hormone status on expression of genes encoding G protein subunits in the rat heart
AU - Levine, Michael A.
AU - Feldman, Arthur M.
AU - Robishaw, Janet D.
AU - Ladenson, Paul W.
AU - Ahn, Thomas G.
AU - Moroney, John F.
AU - Smallwood, Philip M.
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1990/2/25
Y1 - 1990/2/25
N2 - We have studied the influence of thyroid hormone status in vivo on expression of the genes encoding guanine nucleotide-binding regulatory protein (G protein) α-subunits G8α, Giα(2), Giα(3), and both the 36-kDa form (β1) and the 35-kDa form (β2) of the β-subunit in rat ventricle. The relative amounts of immunoactive Giα(2) and Giα(3) were greater in ventricular membranes from hypothyroid animals than from euthyroid animals (1.9- and 2.6-fold, respectively). A corresponding 2.3-fold increase in Giα(2) mRNA was ob-served as well as a 1.5-fold increase in Giα(3) mRNA. The relative amounts of immunoactive β1 and β2 polypeptides were also increased (2.8- and 1.8-fold, respectively) in the hypothyroid state and corresponded with comparable increases in the relative levels of β1 and β2 mRNAs. No difference was seen between the amounts of Giα(2), Giα(3), β1, and β2 in the euthyroid state and the hyperthyroid state. In contrast to these effects of thyroid hormone status on Giα and β, the steady-state amounts of Giα protein and mRNA were not altered by thyroid hormone status. Thyroid hormone status did not alter sensitivity of adenylyl cyclase to stimulation by sodium fluoride or guanyl-5′-yl imidodiphosphate (GppNHp), nor did it influence GppNHp-induced inhibition of forskolin-stimulated enzyme activity. These results demonstrate that thyroid hormone status in vivo can regulate expression of specific G protein subunits in rat myocardium. However, the physiological consequences of these changes remain unclear.
AB - We have studied the influence of thyroid hormone status in vivo on expression of the genes encoding guanine nucleotide-binding regulatory protein (G protein) α-subunits G8α, Giα(2), Giα(3), and both the 36-kDa form (β1) and the 35-kDa form (β2) of the β-subunit in rat ventricle. The relative amounts of immunoactive Giα(2) and Giα(3) were greater in ventricular membranes from hypothyroid animals than from euthyroid animals (1.9- and 2.6-fold, respectively). A corresponding 2.3-fold increase in Giα(2) mRNA was ob-served as well as a 1.5-fold increase in Giα(3) mRNA. The relative amounts of immunoactive β1 and β2 polypeptides were also increased (2.8- and 1.8-fold, respectively) in the hypothyroid state and corresponded with comparable increases in the relative levels of β1 and β2 mRNAs. No difference was seen between the amounts of Giα(2), Giα(3), β1, and β2 in the euthyroid state and the hyperthyroid state. In contrast to these effects of thyroid hormone status on Giα and β, the steady-state amounts of Giα protein and mRNA were not altered by thyroid hormone status. Thyroid hormone status did not alter sensitivity of adenylyl cyclase to stimulation by sodium fluoride or guanyl-5′-yl imidodiphosphate (GppNHp), nor did it influence GppNHp-induced inhibition of forskolin-stimulated enzyme activity. These results demonstrate that thyroid hormone status in vivo can regulate expression of specific G protein subunits in rat myocardium. However, the physiological consequences of these changes remain unclear.
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M3 - Article
C2 - 1689305
AN - SCOPUS:0025231820
SN - 0021-9258
VL - 265
SP - 3553
EP - 3560
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 6
ER -