Influence of the cytosolic carboxy termini of human bl and b2 kinin receptors on ligand internalization

A. Faussner, D. Proud, M. Towns, J. Bathon

Research output: Contribution to journalArticle

Abstract

To examine the role of the cytoplasmic carboxy termini of human B1 and B2 kinin receptors (B1R and B2R) in the internalization of their respective ligands, des-ArglO-kallidin (DAK) and bradykinin (BK), both wild-type receptors, as well as a truncated B2R and chimeric receptors, were expressed as stable clones in CHO cells. Incubation of [3H]-BK at 37°C with cells expressing wild type B2R (Kd = 2nM) resulted in a pronounced and rapid ligand internalization (-80% after 10 min). By contrast incubation of [3H]DAK with cells expressing B1R (Kd = 0.4 nM) resulted in a modest, slow internalization of the ligand (

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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