Influence of the cytosolic carboxy termini of human bl and b2 kinin receptors on ligand internalization

A. Faussner, D. Proud, M. Towns, J. Bathon

Research output: Contribution to journalArticlepeer-review

Abstract

To examine the role of the cytoplasmic carboxy termini of human B1 and B2 kinin receptors (B1R and B2R) in the internalization of their respective ligands, des-ArglO-kallidin (DAK) and bradykinin (BK), both wild-type receptors, as well as a truncated B2R and chimeric receptors, were expressed as stable clones in CHO cells. Incubation of [3H]-BK at 37°C with cells expressing wild type B2R (Kd = 2nM) resulted in a pronounced and rapid ligand internalization (-80% after 10 min). By contrast incubation of [3H]DAK with cells expressing B1R (Kd = 0.4 nM) resulted in a modest, slow internalization of the ligand (

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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