Influence of preapheresis clinical factors on the efficiency of CD34+ cell collection by large-volume apheresis

C. Sarkodee-Adoo, I. Taran, C. Guo, F. Buadi, R. Murthy, E. Cox, R. Lopez, S. Westphal, S. Shope, B. O’connell, L. Wethers, B. Meisenberg

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated 120 leukapheresis procedures (93 patients), in order to detect clinical factors that influence the efficiency of CD34+ collection using Cobe Spectra™ cell separators. Hematocrit was >27% and platelet count >30 000/μl in >95% of patients. Platelet transfusions were given if the postprocedure count was <20 000/μl. Multiple regression analysis was used to analyze putative factors, and a predictive equation defined by stepwise regression modeling. The mean efficiency was 0.59 (s.d. 0.27). Sex (M > F; P = 0.01), the volume processed (inversely; P = 0.01) and CD34+ cell count (inversely; P = 0.04) were associated with efficiency, whereas hematocrit, platelet or leukocyte count, catheter type and patient weight were not. The effect size for predictive factors was small (R2= 0.21). Adverse events were limited to hypocalcemia. We conclude that female sex, volume processed and CD34+ cell count adversely influence the efficiency of CD34+ cell leukapheresis. However, the impact of volume and CD34+ cell count is small, and likely to be offset by the influence of these same factors on overall yield. Leukapheresis appears to be safe and efficient for autologous blood and marrow transplantation patients with hematocrit >27% and platelet count >30 000/μl.

Original languageEnglish (US)
Pages (from-to)851-855
Number of pages5
JournalBone marrow transplantation
Volume31
Issue number10
DOIs
StatePublished - 2003

Keywords

  • CD 34
  • Cobe
  • Efficiency
  • Leukapheresis

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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