TY - JOUR
T1 - Influence of epinephrine on alcohol dehydrogenase activity in rat hepatocyte culture
AU - Mezey, Esteban
AU - Potter, James J.
AU - Litt, Marc R.
AU - Rhodes, Deborah
N1 - Funding Information:
Acknowledgemenfs-This study was supported by Grant AA 00626 from the United States Public Health Service and the Alcoholic Beverage Medical Research Foundation. The authors thank Dr. WGlfgang Vogel of the Department of Pharmacology of the Thomas Jefferson University, Philadelphia, PA, for performing the epinephrine assays.
PY - 1988/8/1
Y1 - 1988/8/1
N2 - The effects of epinephrine on alcohol dehydrogenase activity and on rates of ethanol elimination were determined in rat hepatocyte culture. Continuous exposure of the hepatocytes to epinephrine (10 μM) in combination with dexamethasone (0.1 μM) enhanced alcohol dehydrogenase activity on days 4-7 of culture, whereas neither hormone alone had an effect. The increased alcohol dehydrogenase activity was associated with an increased rate of ethanol elimination. Acute addition of 10 μM epinephrine to hepatocytes maintained in culture with 0.1 μM dexamethasone did not change alcohol dehydrogenase activity, but resulted in an immediate marked, but transitory, increase in ethanol elimination within the first 5 min after the addition of the hormone. Prazosin, an α1-adrenergic blocker, and antimycin, an inhibitor of mitochondrial respiration, were powerful inhibitors of the transient increase in ethanol elimination, whereas 4-methylpyrazole was only partially inhibitory. These observations indicate that epinephrine has a chronic effect in increasing alcohol dehydrogenase activity and ethanol elimination and, also, an acute transient effect of increasing ethanol elimination which is not limited by alcohol dehydrogenase activity.
AB - The effects of epinephrine on alcohol dehydrogenase activity and on rates of ethanol elimination were determined in rat hepatocyte culture. Continuous exposure of the hepatocytes to epinephrine (10 μM) in combination with dexamethasone (0.1 μM) enhanced alcohol dehydrogenase activity on days 4-7 of culture, whereas neither hormone alone had an effect. The increased alcohol dehydrogenase activity was associated with an increased rate of ethanol elimination. Acute addition of 10 μM epinephrine to hepatocytes maintained in culture with 0.1 μM dexamethasone did not change alcohol dehydrogenase activity, but resulted in an immediate marked, but transitory, increase in ethanol elimination within the first 5 min after the addition of the hormone. Prazosin, an α1-adrenergic blocker, and antimycin, an inhibitor of mitochondrial respiration, were powerful inhibitors of the transient increase in ethanol elimination, whereas 4-methylpyrazole was only partially inhibitory. These observations indicate that epinephrine has a chronic effect in increasing alcohol dehydrogenase activity and ethanol elimination and, also, an acute transient effect of increasing ethanol elimination which is not limited by alcohol dehydrogenase activity.
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U2 - 10.1016/0006-2952(88)90287-0
DO - 10.1016/0006-2952(88)90287-0
M3 - Article
C2 - 3395374
AN - SCOPUS:0023894820
VL - 37
SP - 2993
EP - 3000
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 15
ER -