Influence of dose and schedule on the therapeutic efficacy of 131I-labelled monoclonal antibody 139H2 in a human ovarian cancer xenograft model

C. F M Molthoff, H. M. Pinedo, H. M M Schluper, E. Boven

Research output: Contribution to journalArticle

Abstract

The therapeutic efficacy of various doses and schedules of 131I-labelled anti-episialin monoclonal antibody (MAb) 139H2 was assessed in the NIH:OVCAR-3 human ovarian cancer xenograft model. Radioimmunotherapy was started at the time s.c. tumors were well established (100 to 300 mm3). The anti-tumor effects induced by i.v. injections of 131I-MAb 139H2 were dose- and schedule-dependent. Optimal growth inhibition and long-lasting complete tumor regressions were obtained with 2 injections of 500, 700 or 750 μCi 131I-MAb 139H2 per mouse given with a 2-week interval. The percentage of tumors with more than 50% reduction of their initial volume after treatment with a total dose of 1,000 μCi 131I-MAb 139H2 per mouse, given as 10 injections of 100 μCi (3 x/week), 4 injections of 250 μCi (2 x/week), 10 injections of 100 μCi (5 x/week) within a period of 3 weeks, or 2 injections of 500 μCi with a 2-week interval, was 9%, 40%, 64% and 75% respectively. Unlabelled MAb 139H2 did not affect tumor growth, while the effects of 131I-control MAb were minor and transient. 131I-MAb 139H2 treatment did not select for outgrowth of episialin-negative cells in the OVCAR-3 xenografts. Highest absorbed doses of whole-body-radiation were calculated for 2 injections (500 to 750 μCi 131I-MAb 139H2) given with the 2-week interval. The radiation dose to the tumor after a single injection of 500 μCi 131I-MAb 139H2 was 1,300 cGy over 7 days, which appeared slightly lower than the dose calculated after administration of a tracer dose of iodinated MAb 139H2.

Original languageEnglish (US)
Pages (from-to)474-480
Number of pages7
JournalInternational Journal of Cancer
Volume50
Issue number3
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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