Influence of collagen source on fibrillar architecture and properties of vitrified collagen membranes

Shoumyo Majumdar, Qiongyu Guo, Marcos Garza-Madrid, Xiomara Calderon-Colon, Derek Duan, Priscilla Carbajal, Oliver Schein, Morgana Trexler, Jennifer Elisseeff

Research output: Contribution to journalArticle

Abstract

Collagen vitrigel membranes are transparent biomaterials characterized by a densely organized, fibrillar nanostructure that show promise in the treatment of corneal injury and disease. In this study, the influence of different type I collagen sources and processing techniques, including acid-solubilized collagen from bovine dermis (Bov), pepsin-solubilized collagen from human fibroblast cell culture (HuCC), and ficin-solubilized collagen from recombinant human collagen expressed in tobacco leaves (rH), on the properties of the vitrigel membranes was evaluated. Postvitrification carbodiimide crosslinking (CX) was also carried out on the vitrigels from each collagen source, forming crosslinked counterparts BovXL, HuCCXL, and rHXL, respectively. Collagen membrane ultrastructure and biomaterial properties were found to rely heavily on both collagen source and crosslinking. Bov and HuCC samples showed a random fibrillar organization of collagen, whereas rH vitrigels showed remarkable regional fibril alignment. After CX, light transmission was enhanced in all groups. Denaturation temperatures after CX increased in all membranes, of which the highest increase was seen in rH (14.71C), suggesting improved thermal stability of the collagen fibrils in the membranes. Noncrosslinked rH vitrigels may be reinforced through CX to reach levels of mechanical strength and thermal stability comparable to Bov.

Original languageEnglish (US)
Pages (from-to)300-307
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume104
Issue number2
DOIs
StatePublished - Feb 1 2016

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Keywords

  • cornea/conjunctiva
  • extracellular matrix
  • eye
  • ocular

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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