Influence of apical fluid volume on the development of functional intercellular junctions in the human epithelial cell line 16HBE14o-: Implications for the use of this cell line as an in vitro model for bronchial drug absorption studies

Carsten Ehrhardt, Carsten Kneuer, Jennifer Fiegel, Justin Hanes, Ulrich Schaefer, Kwang Jin Kim, Claus Michael Lehr

Research output: Contribution to journalArticle

Abstract

Air-interfaced culture (AIC) versus liquid-covered culture (LCC) conditions are known to have different effects on the differentiated phenotype of several cell types, including lung epithelial cells. We report the influence of culture conditions such as apical medium volume on the development of intercellular junctions in the human epithelial cell line 16HBE14o-. Immunofluorescence staining of the tight-junctional protein, ZO-1, has revealed its presence in cells grown in both AIC and LCC. However, only LCC-grown cells exhibit protein ZO-1 localized as a zonula-occludens-like regular belt connecting neighboring cells. The presence of typical tight junctions has been confirmed by electron microscopy. Immunostaining for occludin, claudin-1, connexin43, and E-cadherin has demonstrated intercellular junction structures only in the cells in LCC. These morphological findings have been paralleled by higher transepithelial electrical resistance values and similar fluxes of the hydrophilic permeability marker, fluorescein-Na, under LCC compared with AIC conditions. We conclude that the formation of functional 16HBE14o- cell layers requires the presence of an apical fluid volume, in contrast to other culture conditions for airway epithelial cells.

Original languageEnglish (US)
Pages (from-to)391-400
Number of pages10
JournalCell and Tissue Research
Volume308
Issue number3
DOIs
StatePublished - Jul 2 2002

Keywords

  • Air-interface
  • Claudin-1
  • Connexin
  • E-cadherin
  • Human epithelial cell line (16HBE14o-)
  • Intercellular junctions
  • Occludin
  • ZO-1

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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