Inflammatory and Microbiologic Markers in Induced Sputum after Intravenous Antibiotics in Cystic Fibrosis

Claudia L. Ordoñez, Noreen R. Henig, Nicole Mayer-Hamblett, Frank J. Accurso, Jane L. Burns, James F. Chmiel, Cori L. Daines, Ronald L. Gibson, Sharon McNamara, George Z. Retsch-Bogart, Pamela L. Zeitlin, Moira L. Aitken

Research output: Contribution to journalArticle

Abstract

Induced sputum has been used to study airway Inflammation. We sought to determine whether markers of Infection and inflammation in induced sputum were a useful and safe outcome measure in cystic fibrosis. We hypothesized that bacterial density and inflammatory content of induced sputum would decrease after antibiotic therapy. Induced sputum was assayed for bacterial density, cell count, and differential and inflammatory markers before and after treatment with intravenous antibiotics. Fifty-five of the 72 subjects enrolled (mean age ± SD 18.2 ± 7.9 years) completed the study. FEV 1 increased by an average 0.3 ± 0.3 L (10.4 ± 8.7% predicted FEV1), p <0.0001; density of Pseudomonas aeruginosa and Staphylococcus aureus decreased by 2.4 ± 3.1 log10 cfu/g (p <0.0005) and 4.0 ± 2.3 log10 cfu/ml (p <0.0001), respectively; neutrophil count decreased by 0.4 ± 0.6 log10 cells/ml (p <0.0001), interleukin-8 concentration by 0.5 ± 1.3 log10 pg/ml (p <0.05), and neutrophil elastase by 0.4 ± 0.7 log10 μg/ml (p <0.005). Seven of 127 (6%) sputum induction procedures showed a decrease in FEV1 of 20% or more. We conclude that markers in induced sputum may be useful, noninvasive outcome measures to assess response to therapies in cystic fibrosis studies.

Original languageEnglish (US)
Pages (from-to)1471-1475
Number of pages5
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume168
Issue number12
DOIs
Publication statusPublished - Dec 15 2003
Externally publishedYes

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Keywords

  • Bacterial density
  • Cystic fibrosis
  • Induced sputum
  • Inflammatory markers

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Ordoñez, C. L., Henig, N. R., Mayer-Hamblett, N., Accurso, F. J., Burns, J. L., Chmiel, J. F., ... Aitken, M. L. (2003). Inflammatory and Microbiologic Markers in Induced Sputum after Intravenous Antibiotics in Cystic Fibrosis. American Journal of Respiratory and Critical Care Medicine, 168(12), 1471-1475. https://doi.org/10.1164/rccm.200306-731OC