Inflammation is a component of neurodegeneration in response to Venezuelan equine encephalitis virus infection in mice

Bruce Schoneboom, Kristen M.K. Catlin, Aileen M. Marty, Franziska B. Grieder

Research output: Contribution to journalArticle

Abstract

Infection with the mosquito-transmitted Venezuelan equine encephalitis virus (VEE) causes an acute systemic febrile illness followed by meningoencephalitis. In this communication we characterize the cytokine profile induced in the central nervous system (CNS) in response to virulent or attenuated strains of VEE using RNase Protection Assays. Virulent VEE causes an upregulation of multiple pro-inflammatory genes including inducible nitric oxide synthase (iNOS) and tumor necrosis factor alpha (TNF-α). To determine if iNOS and TNF-α contribute to the neuropathogenesis of VEE infection, iNOS and TNF receptor knockout mice were used in VEE mortality studies and exhibited extended survival times. Finally, CNS tissue sections labeled for VEE antigen, and adjacent sections double-labeled for an astrocyte marker and apoptosis, revealed that apoptosis of neurons occurs not only in areas of the brain positive for VEE-antigen, but also in areas of astrogliosis. These findings suggest that the inflammatory response, which is in part mediated by iNOS and TNF-α, may contribute to neurodegeneration following encephalitic virus infection. (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)132-146
Number of pages15
JournalJournal of Neuroimmunology
Volume109
Issue number2
DOIs
StatePublished - Sep 22 2000
Externally publishedYes

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Venezuelan Equine Encephalitis Viruses
Virus Diseases
Inflammation
Nitric Oxide Synthase Type II
Tumor Necrosis Factor-alpha
Central Nervous System
Apoptosis
Antigens
Nerve Tissue
Meningoencephalitis
Tumor Necrosis Factor Receptors
Ribonucleases
Infectious Encephalitis
Culicidae
Knockout Mice
Astrocytes
Fever
Up-Regulation
Cytokines
Neurons

Keywords

  • Apoptosis
  • Cytokines
  • Inflammation
  • Neurodegeneration
  • Venezuelan equine encephalitis virus (VEE)

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

Cite this

Inflammation is a component of neurodegeneration in response to Venezuelan equine encephalitis virus infection in mice. / Schoneboom, Bruce; Catlin, Kristen M.K.; Marty, Aileen M.; Grieder, Franziska B.

In: Journal of Neuroimmunology, Vol. 109, No. 2, 22.09.2000, p. 132-146.

Research output: Contribution to journalArticle

Schoneboom, Bruce ; Catlin, Kristen M.K. ; Marty, Aileen M. ; Grieder, Franziska B. / Inflammation is a component of neurodegeneration in response to Venezuelan equine encephalitis virus infection in mice. In: Journal of Neuroimmunology. 2000 ; Vol. 109, No. 2. pp. 132-146.
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