Infection with influenza A(H1N1)pdm09 during the first wave of the 2009 pandemic: Evidence from a longitudinal seroepidemiologic study in Dhaka, Bangladesh

Sharifa Nasreen, Mustafizur Rahman, Kathy Hancock, Jacqueline M. Katz, Doli Goswami, Katharine Sturm-Ramirez, Crystal Holiday, Stacie Jefferson, Alicia Branch, David Wang, Vic Veguilla, Marc Alain Widdowson, Alicia M. Fry, W Abdullah Brooks

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Abstract

Background: We determined influenza A(H1N1)pdm09 antibody levels before and after the first wave of the pandemic in an urban community in Dhaka, Bangladesh. Methods: We identified a cohort of households by stratified random sampling. We collected baseline serum specimens during July-August 2009, just prior to the initial wave of the 2009 pandemic in this community and a second specimen during November 2009, after the pandemic peak. Paired sera were tested for antibodies against A(H1N1)pdm09 virus using microneutralization assay and hemagglutinin inhibition (HI) assay. A fourfold increase in antibody titer by either assay with a titer of ≥40 in the convalescent sera was considered a seroconversion. At baseline, an HI titer of ≥40 was considered seropositive. We collected information on clinical illness from weekly home visits. Results: We tested 779 paired sera from the participants. At baseline, before the pandemic wave, 1% overall and 3% of persons >60 years old were seropositive. After the first wave of the pandemic, 211 (27%) individuals seroconverted against A(H1N1)pdm09. Children aged 5-17 years had the highest proportion (37%) of seroconversion. Among 264 (34%) persons with information on clinical illness, 191 (72%) had illness >3 weeks prior to collection of the follow-up sera and 73 (38%) seroconverted. Sixteen (22%) of these 73 seroconverted participants reported no clinical illness. Conclusion: After the first pandemic wave in Dhaka, one in four persons were infected by A(H1N1)pdm09 virus and the highest burden of infection was among the school-aged children. Seroprevalence studies supplement traditional surveillance systems to estimate infection burden.

Original languageEnglish (US)
Pages (from-to)394-398
Number of pages5
JournalInfluenza and other Respiratory Viruses
Volume11
Issue number5
DOIs
StatePublished - Sep 1 2017

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Keywords

  • Bangladesh
  • H1N1 subtype
  • influenza A virus
  • pandemic
  • seroconversion
  • seroepidemiologic studies

ASJC Scopus subject areas

  • Epidemiology
  • Pulmonary and Respiratory Medicine
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Nasreen, S., Rahman, M., Hancock, K., Katz, J. M., Goswami, D., Sturm-Ramirez, K., Holiday, C., Jefferson, S., Branch, A., Wang, D., Veguilla, V., Widdowson, M. A., Fry, A. M., & Brooks, W. A. (2017). Infection with influenza A(H1N1)pdm09 during the first wave of the 2009 pandemic: Evidence from a longitudinal seroepidemiologic study in Dhaka, Bangladesh. Influenza and other Respiratory Viruses, 11(5), 394-398. https://doi.org/10.1111/irv.12462