Infection of laboratory colonies of Anopheles mosquitoes with Plasmodium vivax from cryopreserved clinical isolates

Kathryn Shaw-Saliba, David Clarke, Jorge M. Santos, Maria José Menezes, Caeul Lim, Anjali Mascarenhas, Laura Chery, Edwin Gomes, Sandra March, Sangeeta N. Bhatia, Pradipsinh K. Rathod, Marcelo U. Ferreira, Flaminia Catteruccia, Manoj T. Duraisingh

Research output: Contribution to journalArticle

Abstract

Plasmodium vivax is the most geographically widespread malaria parasite. Unique features of transmission biology complicate P. vivax control. Interventions targeting transmission are required for malaria eradication. In the absence of an in vitro culture, transmission studies rely on live isolates from non-human primates or endemic regions. Here, we demonstrate P. vivax gametocytes from both India and Brazil are stable during cryopreservation. Importantly, cryopreserved gametocytes from Brazil were capable of infecting three anopheline mosquito species in feedings done in the United States. These findings create new opportunities for transmission studies in diverse locales.

Original languageEnglish (US)
Pages (from-to)679-683
Number of pages5
JournalInternational Journal for Parasitology
Volume46
Issue number11
DOIs
StatePublished - Oct 1 2016

Keywords

  • Anopheles
  • Cryopreservation
  • Gametocyte
  • Liver
  • Malaria
  • Plasmodium vivax
  • Sporozoite
  • Transmission

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Infection of laboratory colonies of Anopheles mosquitoes with Plasmodium vivax from cryopreserved clinical isolates'. Together they form a unique fingerprint.

  • Cite this

    Shaw-Saliba, K., Clarke, D., Santos, J. M., Menezes, M. J., Lim, C., Mascarenhas, A., Chery, L., Gomes, E., March, S., Bhatia, S. N., Rathod, P. K., Ferreira, M. U., Catteruccia, F., & Duraisingh, M. T. (2016). Infection of laboratory colonies of Anopheles mosquitoes with Plasmodium vivax from cryopreserved clinical isolates. International Journal for Parasitology, 46(11), 679-683. https://doi.org/10.1016/j.ijpara.2016.06.003