Infection of human amniotic and endothelial cells by Japanese encephalitis virus: Increased expression of HLA-F

Gaurav Kumar, Onkar Sanjay Date, Kwang Sik Kim, Ramanathapuram Manjunath

Research output: Contribution to journalArticlepeer-review

Abstract

Productive infection of human amniotic and endothelial cell lines with Japanese encephalitis virus (JEV) was established leading to the induction of NFκB and HLA-F, a non-classical MHC molecule. Induction of the HLA-F gene and protein in JEV-infected cells was shown to be NFκB dependent since it was blocked by inhibitors of NFκB activation. ShRNA targeting lentivirus-mediated stable knockdown of the p65 subunit of NFκB inhibited JEV-mediated induction of HLA-F both in the amniotic cell line, AV-3 as well as the human brain microendothelial cell line, HBMEC. The induction of HLA-F by treatment of AV-3 with TNF-α was also inhibited by ShRNA mediated knockdown of NFκB. TNF-α treatment of HEK293T cells that were transfected with reporter plasmids under the control of HLA-F enhancer A elements resulted in significant transactivation of the luciferase reporter gene. NFκB-mediated induction of HLA-F following JEV infection and TNF-α exposure is being suggested for the first time.

Original languageEnglish (US)
Pages (from-to)29-37
Number of pages9
JournalVirology
Volume471
DOIs
StatePublished - Dec 1 2014

Keywords

  • Amniotic
  • Endothelial cells
  • JEV
  • NFκB mediated HLA-F induction
  • TNF-α

ASJC Scopus subject areas

  • Virology

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