Infection during natural orifice transluminal endoscopic surgery: a randomized, controlled study in a live porcine model

Samuel A. Giday, Xavier Dray, Priscilla Magno, Jonathan M. Buscaglia, Eun Ji Shin, Vihar C. Surti, Lia Assumpcao, Michael R. Marohn, Dawn Ruben, Andrew Zhigalin, Laurie J. Pipitone, Sergey V. Kantsevoy

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Currently reported natural orifice transluminal endoscopic surgery (NOTES) procedures in animals have been done in heterogeneous milieus ranging from nonsterile to sterile procedures, with mixed results, including no infection in those performed in nonsterile settings. Objective: To establish the potential frequency of infection during NOTES, comparing sterile to nonsterile approaches. Setting: Survival experiments on sixteen 50-kg pigs. Design and Interventions: From pilot data (100% infection frequency after nonsterile procedures), sample size (8 animals in each group) was calculated by using a power of 95% and an alpha risk of 0.05. The animals were randomly assigned to two groups: In the transgastric peritoneoscopy study group, liver and ovarian biopsies were performed with sterile overtubes, endoscopes, and accessories and the use of preoperative intravenous antibiotics and antiseptic gastric lavage. In the nonsterile (control) group, the same procedures were performed with nonsterile endoscopes and accessories without the use of gastric lavage and preoperative antibiotics. Complete transmural closure of the transgastric access site was made in all animals. After a 1-week survival time, all animals were killed for necropsy, which included Gram staining and peritoneal cultures. Main Outcome Measurements: Intraperitoneal infection on necropsy. Results: All necropsies revealed intraperitoneal infection (abscesses, fibrinopurulent exudates, and adhesions) in the control group (frequency of infection 100%). Peritoneal bacterial culture grew various aerobic and anaerobic organisms. No gross or bacteriological evidence of infection was seen in the sterile group (frequency of infection 0%, P value = <.0002). Limitations: Animal experiments. Conclusion: Nonsterile conditions invariably lead to intraperitoneal infection. Aseptic techniques during NOTES can prevent intra-abdominal infection. Future studies will determine which infection prevention steps are mandatory and which can be omitted during NOTES procedures.

Original languageEnglish (US)
Pages (from-to)812-816
Number of pages5
JournalGastrointestinal endoscopy
Volume71
Issue number4
DOIs
StatePublished - Apr 2010

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Gastroenterology

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