Induction of tolerance in a rat model of laryngeal transplantation

Lee M. Akst, Maria Siemionow, Olivia Dan, Dariusz Izycki, Marshall Strome

Research output: Contribution to journalArticlepeer-review

Abstract

Background. The major limitation preventing expansion of laryngeal transplantation as a therapeutic modality is the necessity of lifelong immunosuppression. In this report, we describe an immunomodulatory strategy for tolerance induction in laryngeal allotransplantation that permits escape from chronic immunosuppression. Materials and Methods. Larynges were transplanted from Lewis-Brown-Norway (RT11/n, F1) donors to Lewis (RT1 1) recipients. Recipients received 7 days of treatment with tacrolimus and mouse anti-rat αβ T-cell-receptor (TCR) monoclonal antibodies. Histology, mixed lymphocyte reaction (MLR), skin grafting, and flow cytometry assessed functional tolerance, efficacy of immunodepletion, and donor-specific chimerism. Results. All 10 recipients survived until sacrifice at 100 days. Histology suggested functional allograft tolerance. Skin grafting, MLR, and flow cytometry revealed that tolerance is neither donor-specific nor related to systemic immunocompromise. Conclusions. In this rat laryngeal-transplantation model, functional tolerance was induced under combined tacrolimus and αβ TCR protocol. Mechanisms responsible for this tolerance induction require future elucidation.

Original languageEnglish (US)
Pages (from-to)1763-1766
Number of pages4
JournalTransplantation
Volume76
Issue number12
DOIs
StatePublished - Dec 27 2003
Externally publishedYes

ASJC Scopus subject areas

  • Transplantation

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