Induction of the transcription factors NF-κB, AP-1 and NF-AT during B cell stimulation through the CD40 receptor

Delicia A. Francis, James G. Karras, Xiao yan Ke, Ranjan Sen, Thomas L. Rothstein

Research output: Contribution to journalArticle

Abstract

To address elements that might uniquely characterize CD40 mediated signaling, the nuclear expression of three transcription factors was evaluated following B cell stimulation by CD40L and by anti-lg antibody. Cross-linked CD40L was found to induce nuclear expression of NF-k{cyrillic}B, AP-1 and NF-AT with a time course and intensity similar to that produced by anti-lg. Examination of NF-k{cyrillic}B in more detail demonstrated that the CD40 mediated expression of DNA binding complexes correlated with induction of trans-activating activity which again attained similar levels following cross-linking of CD40 and slg. Despite the marked similarity In transcription factor induction triggered through CD40 and slg, differences in the Intracellular signaling pathways utilized were apparent in that protein kinase C (PKC) depletion did not affect CD40 mediated induction of NF-k{cyrillic}B even as induction by anti-lg was abolished. These results suggest that a 'final common pathway'or convergence of transcription factor induction may exist for two distinct receptors, each of which is individually capable of triggering cell cycle progression, despite the use of separate intracellular signaling pathways that differ at the level of PKC. Although transcription factor induction was similar for CD40L and anti-lg early on, subtle differences In expressed NF-k{cyrillic}B and AP-1 nucleoprotein complexes were apparent at 24 h. Such differences may play a role in determining the variant effects on B cells of stimulation through these two receptors.

Original languageEnglish (US)
Pages (from-to)151-161
Number of pages11
JournalInternational Immunology
Volume7
Issue number2
DOIs
StatePublished - Feb 1995
Externally publishedYes

Fingerprint

Transcription factors
B Cells
Transcription Factor AP-1
Transcription Factor
Receptor
CD40 Ligand
Proof by induction
B-Lymphocytes
Transcription Factors
Cells
Protein Kinase C
Signaling Pathways
Proteins
Nucleoproteins
Antibodies
Anti-Idiotypic Antibodies
Cell Cycle
DNA
Depletion
Progression

Keywords

  • CD40 ligand
  • DNA binding protiens
  • Nucleoprotien complexes
  • Proliferation
  • Protien kinase C
  • Surface lg

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation
  • Immunology

Cite this

Induction of the transcription factors NF-κB, AP-1 and NF-AT during B cell stimulation through the CD40 receptor. / Francis, Delicia A.; Karras, James G.; Ke, Xiao yan; Sen, Ranjan; Rothstein, Thomas L.

In: International Immunology, Vol. 7, No. 2, 02.1995, p. 151-161.

Research output: Contribution to journalArticle

Francis, Delicia A. ; Karras, James G. ; Ke, Xiao yan ; Sen, Ranjan ; Rothstein, Thomas L. / Induction of the transcription factors NF-κB, AP-1 and NF-AT during B cell stimulation through the CD40 receptor. In: International Immunology. 1995 ; Vol. 7, No. 2. pp. 151-161.
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