Induction of the Intercellular Adhesion Molecule (ICAM-1) on Squamous Cell Carcinoma by Interferon Gamma

Richard L. Scher, Wayne Martin Koch, William J. Richtsmeier

Research output: Contribution to journalArticle

Abstract

To determine if the cell surface antigen, the intercellular adhesion molecule 1 (ICAM-1), is expressed on head and neck (H&N) squamous cell carcinoma (SCC) cell lines, and if treatment with interferon gamma (IFN-γ) enhances the expression of the antigen. Intercellular adhesion molecule 1 mediates effector cell adhesion, activation, and function in inflammatory and immunologic reactions, and it may be important in the generation of antitumor immune surveillance and cytotoxicity against H&N SCC. —Four human SCC cell lines, JHU-011-SCC, JHU-020-SCC, JHU-022-SCC, and FaDu, established by explant technique from tumors of the upper aerodigestive tract, were utilized for these experiments. The cell lines were maintained and tested under standard tissue culture conditions. —Fluorescence-activated cell sorting and enzyme-linked immunosorbent assay were performed to identify the presence of ICAM-1 on the H&N SCC cell lines after staining with an anti—ICAM-1 monoclonal antibody (CD54). The SCC cell lines were treated with either fresh media or varying dosages (1 to 1000 U/mL) of recombinant human interferon gamma (rHuIFN-γ) to determine constitutive and enhanced antigen expression. The kinetics of the response to rHuIFN-γ were determined for the JHU-022-SCC cell line. The effect of the cytokines interleukin 1, interleukin 2, tumor necrosis factor α, and interferon alfa on ICAM-1 expression on JHU-022-SCC was also tested. —Constitutive and enhanced ICAM-1 expression. —Low levels of constitutive expression of ICAM-1 were identified on all four H&N SCC cell lines, with significantly enhanced expression seen after rHuIFN-γ treatment (P=.0001). Maximally enhanced expression of the antigen on JHU-022-SCC occurred after treatment for 48 hours with 100 U/mL of rHuIFN-γ (P =.0001). Induction of ICAM-1 expression was detectable after treatment with as little as 10 U/mL of rHuIFN-γ (P

Original languageEnglish (US)
Pages (from-to)432-438
Number of pages7
JournalArchives of Otolaryngology--Head and Neck Surgery
Volume119
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Cell Adhesion Molecules
Intercellular Adhesion Molecule-1
Interferon-gamma
Squamous Cell Carcinoma
Cell Line
Antigens
Therapeutics
Surface Antigens
Interleukin-1
Interferon-alpha
Cell Adhesion
Interleukin-2
Flow Cytometry
Neck
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Head
Monoclonal Antibodies
human IFNG protein
Staining and Labeling

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery

Cite this

Induction of the Intercellular Adhesion Molecule (ICAM-1) on Squamous Cell Carcinoma by Interferon Gamma. / Scher, Richard L.; Koch, Wayne Martin; Richtsmeier, William J.

In: Archives of Otolaryngology--Head and Neck Surgery, Vol. 119, No. 4, 1993, p. 432-438.

Research output: Contribution to journalArticle

@article{947f8b4cc9ef4ddeb3c22dde5d148308,
title = "Induction of the Intercellular Adhesion Molecule (ICAM-1) on Squamous Cell Carcinoma by Interferon Gamma",
abstract = "To determine if the cell surface antigen, the intercellular adhesion molecule 1 (ICAM-1), is expressed on head and neck (H&N) squamous cell carcinoma (SCC) cell lines, and if treatment with interferon gamma (IFN-γ) enhances the expression of the antigen. Intercellular adhesion molecule 1 mediates effector cell adhesion, activation, and function in inflammatory and immunologic reactions, and it may be important in the generation of antitumor immune surveillance and cytotoxicity against H&N SCC. —Four human SCC cell lines, JHU-011-SCC, JHU-020-SCC, JHU-022-SCC, and FaDu, established by explant technique from tumors of the upper aerodigestive tract, were utilized for these experiments. The cell lines were maintained and tested under standard tissue culture conditions. —Fluorescence-activated cell sorting and enzyme-linked immunosorbent assay were performed to identify the presence of ICAM-1 on the H&N SCC cell lines after staining with an anti—ICAM-1 monoclonal antibody (CD54). The SCC cell lines were treated with either fresh media or varying dosages (1 to 1000 U/mL) of recombinant human interferon gamma (rHuIFN-γ) to determine constitutive and enhanced antigen expression. The kinetics of the response to rHuIFN-γ were determined for the JHU-022-SCC cell line. The effect of the cytokines interleukin 1, interleukin 2, tumor necrosis factor α, and interferon alfa on ICAM-1 expression on JHU-022-SCC was also tested. —Constitutive and enhanced ICAM-1 expression. —Low levels of constitutive expression of ICAM-1 were identified on all four H&N SCC cell lines, with significantly enhanced expression seen after rHuIFN-γ treatment (P=.0001). Maximally enhanced expression of the antigen on JHU-022-SCC occurred after treatment for 48 hours with 100 U/mL of rHuIFN-γ (P =.0001). Induction of ICAM-1 expression was detectable after treatment with as little as 10 U/mL of rHuIFN-γ (P",
author = "Scher, {Richard L.} and Koch, {Wayne Martin} and Richtsmeier, {William J.}",
year = "1993",
doi = "10.1001/archotol.1993.01880160080012",
language = "English (US)",
volume = "119",
pages = "432--438",
journal = "Archives of Otolaryngology",
issn = "2168-6181",
publisher = "American Medical Association",
number = "4",

}

TY - JOUR

T1 - Induction of the Intercellular Adhesion Molecule (ICAM-1) on Squamous Cell Carcinoma by Interferon Gamma

AU - Scher, Richard L.

AU - Koch, Wayne Martin

AU - Richtsmeier, William J.

PY - 1993

Y1 - 1993

N2 - To determine if the cell surface antigen, the intercellular adhesion molecule 1 (ICAM-1), is expressed on head and neck (H&N) squamous cell carcinoma (SCC) cell lines, and if treatment with interferon gamma (IFN-γ) enhances the expression of the antigen. Intercellular adhesion molecule 1 mediates effector cell adhesion, activation, and function in inflammatory and immunologic reactions, and it may be important in the generation of antitumor immune surveillance and cytotoxicity against H&N SCC. —Four human SCC cell lines, JHU-011-SCC, JHU-020-SCC, JHU-022-SCC, and FaDu, established by explant technique from tumors of the upper aerodigestive tract, were utilized for these experiments. The cell lines were maintained and tested under standard tissue culture conditions. —Fluorescence-activated cell sorting and enzyme-linked immunosorbent assay were performed to identify the presence of ICAM-1 on the H&N SCC cell lines after staining with an anti—ICAM-1 monoclonal antibody (CD54). The SCC cell lines were treated with either fresh media or varying dosages (1 to 1000 U/mL) of recombinant human interferon gamma (rHuIFN-γ) to determine constitutive and enhanced antigen expression. The kinetics of the response to rHuIFN-γ were determined for the JHU-022-SCC cell line. The effect of the cytokines interleukin 1, interleukin 2, tumor necrosis factor α, and interferon alfa on ICAM-1 expression on JHU-022-SCC was also tested. —Constitutive and enhanced ICAM-1 expression. —Low levels of constitutive expression of ICAM-1 were identified on all four H&N SCC cell lines, with significantly enhanced expression seen after rHuIFN-γ treatment (P=.0001). Maximally enhanced expression of the antigen on JHU-022-SCC occurred after treatment for 48 hours with 100 U/mL of rHuIFN-γ (P =.0001). Induction of ICAM-1 expression was detectable after treatment with as little as 10 U/mL of rHuIFN-γ (P

AB - To determine if the cell surface antigen, the intercellular adhesion molecule 1 (ICAM-1), is expressed on head and neck (H&N) squamous cell carcinoma (SCC) cell lines, and if treatment with interferon gamma (IFN-γ) enhances the expression of the antigen. Intercellular adhesion molecule 1 mediates effector cell adhesion, activation, and function in inflammatory and immunologic reactions, and it may be important in the generation of antitumor immune surveillance and cytotoxicity against H&N SCC. —Four human SCC cell lines, JHU-011-SCC, JHU-020-SCC, JHU-022-SCC, and FaDu, established by explant technique from tumors of the upper aerodigestive tract, were utilized for these experiments. The cell lines were maintained and tested under standard tissue culture conditions. —Fluorescence-activated cell sorting and enzyme-linked immunosorbent assay were performed to identify the presence of ICAM-1 on the H&N SCC cell lines after staining with an anti—ICAM-1 monoclonal antibody (CD54). The SCC cell lines were treated with either fresh media or varying dosages (1 to 1000 U/mL) of recombinant human interferon gamma (rHuIFN-γ) to determine constitutive and enhanced antigen expression. The kinetics of the response to rHuIFN-γ were determined for the JHU-022-SCC cell line. The effect of the cytokines interleukin 1, interleukin 2, tumor necrosis factor α, and interferon alfa on ICAM-1 expression on JHU-022-SCC was also tested. —Constitutive and enhanced ICAM-1 expression. —Low levels of constitutive expression of ICAM-1 were identified on all four H&N SCC cell lines, with significantly enhanced expression seen after rHuIFN-γ treatment (P=.0001). Maximally enhanced expression of the antigen on JHU-022-SCC occurred after treatment for 48 hours with 100 U/mL of rHuIFN-γ (P =.0001). Induction of ICAM-1 expression was detectable after treatment with as little as 10 U/mL of rHuIFN-γ (P

UR - http://www.scopus.com/inward/record.url?scp=0027416637&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027416637&partnerID=8YFLogxK

U2 - 10.1001/archotol.1993.01880160080012

DO - 10.1001/archotol.1993.01880160080012

M3 - Article

C2 - 8096142

AN - SCOPUS:0027416637

VL - 119

SP - 432

EP - 438

JO - Archives of Otolaryngology

JF - Archives of Otolaryngology

SN - 2168-6181

IS - 4

ER -