Induction of sterilizing immunity against west nile virus (WNV), by immunization with WNV-like particles produced in insect cells

Ming Qiao, Mundrigi Ashok, Kristen A. Bernard, Gustavo Palacios, Z. Hong Zhou, W. Ian Lipkin, T. Jake Liang

Research output: Contribution to journalArticlepeer-review

Abstract

No specific vaccine for West Nile virus (WNV) is currently available for human use. In the present study, we describe the generation of WNV-like particles (WNV-LPs) in insect cells by use of recombinant baculoviruses expressing the WNV structural proteins prME or CprME. BALB/c mice immunized with purified WNV-LPs developed WNV-specific antibodies that had potent neutralizing activities. Mice immunized with prME-like particles (prME-LPs) showed no morbidity or mortality after challenge with WNV. Immunization with prME-LPs can induce sterilizing immunity without producing any evidence of viremia or viral RNA in the spleen or brain. These results suggest that WNV-LPs hold promise as a vaccine candidate for WNV infection.

Original languageEnglish (US)
Pages (from-to)2104-2108
Number of pages5
JournalJournal of Infectious Diseases
Volume190
Issue number12
DOIs
StatePublished - Dec 15 2004
Externally publishedYes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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