Induction of sister-chromatid exchanges by polycyclic aromatic hydrocarbons following metabolic activation with phorbol ester-stimulated human polymorphonuclear leukocytes

J. L. Seed, T. W. Kensler, M. Elia, M. A. Trush

Research output: Contribution to journalArticlepeer-review

Abstract

We previously observed that the interaction of an oxidant generated by polymorphonuclear leukocytes (PMNs) with (±) -trans benzo [a]pyrene-7,8-dihydrodiol (BP-7,8-diol) resulted in covalent binding to DNA and elicted bacterial mutagenesis (PNAS 82:5194, 1985). We now report that this interaction also induces sister chromatid exchanges (SCEs) in Chinese hamster V-79 cells. This genotoxic response required stimulation of the PMNs by phorbol ester as no effect was observed with unstimulated cells. Likewise, no intrinsic activity of BP-7,8-diol alone was noted. The addition of azide, CuDIPS, or taurine markedly inhibited the induction of SCEs by the combination of BP-7,8-diol and stimulated PMNs, further suggesting the involvement of myeloperoxidase in the activation of the polycyclic aromatic hydrocarbon. The (-) isomer of BP-7,8-diol as well as 7,8-dihydro-BP were more active than (+)-BP-7,8-diol in inducing SCEs. By contrast, benzo[a]pyrene or derivatives lacking a double bond at the 9,10 position were not effective in inducing SCEs above the level seen with phorbol ester-stimulated PMNs. These observations serve to underscore the potential for myeloperoxidase-dependent activation of xenobiotics by PMNs to result in a localized genotoxic environment.

Original languageEnglish (US)
Pages (from-to)349-360
Number of pages12
JournalResearch communications in chemical pathology and pharmacology
Volume67
Issue number3
StatePublished - 1990

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Toxicology
  • Pharmacology
  • Pharmacology, Toxicology and Pharmaceutics(all)

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