The present study investigated whether a short synthetic peptide NPP, with a modified sequence (147–158 R156−) derived from influenza A virus nucleoprotein with high affinity for Kd major histocompatibillity complex class I molecules, could induce primary influenza virus‐specific cytotoxic T (Tc) cells in vitro. Naive BALB/c (H‐2d) splenocytes did not respond to the stimulation with only NPP with the generation of effector Tc cells specific for influenza A virus‐infected target cells in vitro. However, they were able to do so if cultured with NPP in the presence of IL‐7. IL‐7 activity in this system differed significantly from IL‐2 activity in the specificity of the effect. The use of exogenous IL‐2, instead of IL‐7, with NPP resulted in the induction of lytic cells that lysed both influenza virus‐infected and uninfected syngeneic target cells. These results suggest that IL‐7 is a potent regulatory cytokine in the antigen‐specific activation of resting naive Tc cell precursors and may provide the necessary conditions for the induction of human primary Tc cells in vitro.
ASJC Scopus subject areas
- Immunology and Allergy