Induction of partial chimerism in nonirradiated B-lymphocyte-deficient CBA/N mice

D. Volf, L. L. Sensenbrenner, S. J. Sharkis, G. J. Elfenbein, I. Scher

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Nonirradiated B-lymphocyte-deficient CBA/N mice given T6T6 chromosome marked normal CBA/CaHN spleen cells became lymphoid chimeras exhibiting donor-type mitoses. Normal CBA/CaHN recepients did not exhibit significant numbers of donor-type mitoses. The lymphoid cell chimerism in the CBA/N host appeared in spleen, lymph nodes, and Peyer's patches, but not in bone marrow or thymus. Stimulation of CBA/N-recipient spleen cells in vitro suggested that the chimerism involved donor T6T6 cells which were responsive to the B-lymphocyte mitogen, lipopolysaccharide, but not to the T-lymphocyte mitogen, phytohemagglutinin. These data indicate that stable, long-term chimerism of a specific class of lymphocytes is possible in nonirradiated, B-lymphocyte-deficient CBA/N mice.

Original languageEnglish (US)
Pages (from-to)940-945
Number of pages6
JournalJournal of Experimental Medicine
Issue number3
Publication statusPublished - 1978


ASJC Scopus subject areas

  • Immunology

Cite this

Volf, D., Sensenbrenner, L. L., Sharkis, S. J., Elfenbein, G. J., & Scher, I. (1978). Induction of partial chimerism in nonirradiated B-lymphocyte-deficient CBA/N mice. Journal of Experimental Medicine, 147(3), 940-945.