Induction of myocardial infarcts of a predictable size and location by branch pattern probability-assisted coronary ligation in C57BL/6 mice

Dongchoon Ahn, Linda Cheng, Chanil Moon, Harold Spurgeon, Edward G. Lakatta, Mark I. Talan

Research output: Contribution to journalArticlepeer-review

Abstract

The ability to create experimental myocardial infarctions of reproducible size and location is tantamount to progress in multiple facets of ischemic heart disease research. Branches of the mouse left main descending coronary artery penetrate the myocardium close to their origin and require "blind" ligation. Our objective was to develop a technique for ligation of nonvisible coronary artery branches to permit the reliable creation of infarcts of uniformly small size and location. From latex castings of the left coronary artery of C57BL/6J mice (n = 53), we calculated the highest probability for the location of branch points of two of three left ventricular (LV) branches distal to the origin of the left main descending artery. On the basis of these anatomic probabilities, we blindly ligated two areas that were likely to be locations of these nonvisible LV branches. We were successful in producing two types of small transmural myocardial infarctions (16.04 ± 3.64 and 4.68 ± 1.47% of the LV) in 57% of attempts. Thus our branch pattern probability-assisted method permits routine creation of small infarcts of uniform size in the mouse.

Original languageEnglish (US)
Pages (from-to)H1201-H1207
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume286
Issue number3 55-3
DOIs
StatePublished - Mar 2004
Externally publishedYes

Keywords

  • Anatomy
  • Cardiovascular physiology
  • Heart
  • Method

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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