Induction of Morphologically Abnormal Sperm in Rats Exposed to O-Xylene

W. J. Washington; R. C. Murthy; A. Doye; K. Eugene; D. Brown; I. Bradley

doi:10.3109/01485018308987487

Induction of Morphologically Abnormal Sperm in Rats Exposed to O-Xylene

W. J. Washington, R. C. Murthy, A. Doye, K. Eugene, D. Brown, I. Bradley

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

An analysis of sperm abnormalities can be used as a rapid method to determine the mutagenic potential of chemical agents as well as the toxic potential of chemicals on the whole animal. O-Xylene was investigated for its potential mutagenicity by the sperm abnormality assay. Sprague Dawley rats 10-16 weeks old were injected intraperitoneally (ip) with 0.5 and 1.5 ml/kg body weight of o-xylene in corn oil. Five weeks after treatment, animals were sacrificed and sperm examined for morphological abnormalities. Several morphologically abnormal sperm types were observed; among these were those with amorphous heads, and banana-like heads. Animals housed at room temperatures of 20-24C showed no significant increase in abnormal sperm over the control. Rats housed at temperatures between 24 and 30C after ip injection with 0.5 ml/kg o-xylene showed a significant increase in abnormal sperm.

Original language	English (US)
Pages (from-to)	233-237
Number of pages	5
Journal	Archives of Andrology
Volume	11
Issue number	3
DOIs	https://doi.org/10.3109/01485018308987487
State	Published - Jan 1983
Externally published	Yes

Keywords

Intraperitoneal injection
Mutagenicity
O-Xylene
Sperm abnormalities

ASJC Scopus subject areas

Endocrinology

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10.3109/01485018308987487

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title = "Induction of Morphologically Abnormal Sperm in Rats Exposed to O-Xylene",

abstract = "An analysis of sperm abnormalities can be used as a rapid method to determine the mutagenic potential of chemical agents as well as the toxic potential of chemicals on the whole animal. O-Xylene was investigated for its potential mutagenicity by the sperm abnormality assay. Sprague Dawley rats 10-16 weeks old were injected intraperitoneally (ip) with 0.5 and 1.5 ml/kg body weight of o-xylene in corn oil. Five weeks after treatment, animals were sacrificed and sperm examined for morphological abnormalities. Several morphologically abnormal sperm types were observed; among these were those with amorphous heads, and banana-like heads. Animals housed at room temperatures of 20-24C showed no significant increase in abnormal sperm over the control. Rats housed at temperatures between 24 and 30C after ip injection with 0.5 ml/kg o-xylene showed a significant increase in abnormal sperm.",

keywords = "Intraperitoneal injection, Mutagenicity, O-Xylene, Sperm abnormalities",

author = "Washington, {W. J.} and Murthy, {R. C.} and A. Doye and K. Eugene and D. Brown and I. Bradley",

note = "Funding Information: Acknowledgment: The authors would like to acknowledge the secretarial assistance of VeenaTurner. This \tudy was supported by the National Institute of Health Minority Biomedical Research Support Program Grant Number 2 SO6 RR8052.",

year = "1983",

month = jan,

doi = "10.3109/01485018308987487",

language = "English (US)",

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pages = "233--237",

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AU - Washington, W. J.

AU - Murthy, R. C.

AU - Doye, A.

AU - Eugene, K.

AU - Brown, D.

AU - Bradley, I.

N1 - Funding Information: Acknowledgment: The authors would like to acknowledge the secretarial assistance of VeenaTurner. This \tudy was supported by the National Institute of Health Minority Biomedical Research Support Program Grant Number 2 SO6 RR8052.

PY - 1983/1

Y1 - 1983/1

N2 - An analysis of sperm abnormalities can be used as a rapid method to determine the mutagenic potential of chemical agents as well as the toxic potential of chemicals on the whole animal. O-Xylene was investigated for its potential mutagenicity by the sperm abnormality assay. Sprague Dawley rats 10-16 weeks old were injected intraperitoneally (ip) with 0.5 and 1.5 ml/kg body weight of o-xylene in corn oil. Five weeks after treatment, animals were sacrificed and sperm examined for morphological abnormalities. Several morphologically abnormal sperm types were observed; among these were those with amorphous heads, and banana-like heads. Animals housed at room temperatures of 20-24C showed no significant increase in abnormal sperm over the control. Rats housed at temperatures between 24 and 30C after ip injection with 0.5 ml/kg o-xylene showed a significant increase in abnormal sperm.

AB - An analysis of sperm abnormalities can be used as a rapid method to determine the mutagenic potential of chemical agents as well as the toxic potential of chemicals on the whole animal. O-Xylene was investigated for its potential mutagenicity by the sperm abnormality assay. Sprague Dawley rats 10-16 weeks old were injected intraperitoneally (ip) with 0.5 and 1.5 ml/kg body weight of o-xylene in corn oil. Five weeks after treatment, animals were sacrificed and sperm examined for morphological abnormalities. Several morphologically abnormal sperm types were observed; among these were those with amorphous heads, and banana-like heads. Animals housed at room temperatures of 20-24C showed no significant increase in abnormal sperm over the control. Rats housed at temperatures between 24 and 30C after ip injection with 0.5 ml/kg o-xylene showed a significant increase in abnormal sperm.

KW - Intraperitoneal injection

KW - Mutagenicity

KW - O-Xylene

KW - Sperm abnormalities

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Induction of Morphologically Abnormal Sperm in Rats Exposed to O-Xylene

Abstract

Keywords

ASJC Scopus subject areas

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