Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations

Saraswati Sukumar, Vicente Notario, Dionisio Martin-Zanca, Mariano Barbacid

Research output: Contribution to journalArticle

Abstract

Each of nine mammary carcinomas induced by a single injection of nitroso-methylurea into 50-day-old Buf/N female rats, contained a transforming H-ras-1 gene. Molecular characterization of one of the genes revealed that the twelfth codon was GAA instead of GGA of the normal allele, encoding glutamic acid in place of glycine. These results indicate that chemical carcinogenesis represents an adequate model to study the role of transforming ras genes in human neoplasia.

Original languageEnglish (US)
Pages (from-to)658-661
Number of pages4
JournalNature
Volume306
Issue number5944
DOIs
StatePublished - 1983
Externally publishedYes

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ras Genes
Oncogenes
Point Mutation
Breast Neoplasms
Codon
Glycine
Glutamic Acid
Carcinogenesis
Alleles
Injections
Genes
Neoplasms
methylurea

ASJC Scopus subject areas

  • General

Cite this

Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations. / Sukumar, Saraswati; Notario, Vicente; Martin-Zanca, Dionisio; Barbacid, Mariano.

In: Nature, Vol. 306, No. 5944, 1983, p. 658-661.

Research output: Contribution to journalArticle

Sukumar, Saraswati ; Notario, Vicente ; Martin-Zanca, Dionisio ; Barbacid, Mariano. / Induction of mammary carcinomas in rats by nitroso-methylurea involves malignant activation of H-ras-1 locus by single point mutations. In: Nature. 1983 ; Vol. 306, No. 5944. pp. 658-661.
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