Induction of macrophage Ia antigen expression by rIFN-γ and down-regulation by IFN-α/β and dexamethasone are mediated by changes in steady-state levels of Ia mRNA

Diana Fertsch, D. R. Schoenberg, R. N. Germain, J. Y. Tou, S. N. Vogel

Research output: Contribution to journalArticle

Abstract

In previous studies, the induction of Ia antigens on murine peritoneal exudate macrophages by recombinant IFN-γ (rIFN-γ) and the antagonism of rIFN-γ-induced Ia expression by the inhibitors IFN-α/β and glucocorticoids have been examined. In this report, these findings have been extended to an analysis of total or cytoplasmic mRNA from macrophage cultures treated with rIFN-γ in the absence or presence of these two inhibitors. Recombinant IFN-γ induced a 5.7- to 6.5-fold increase in steady-state levels of Ia (A(α)-specific) mRNA. Coordinate increases in steady-state mRNA for A(β), and E(α) were observed in response to rIFN-γ. Maximum induction occurred 24 hr post-treatment and required the continued presence of rIFN-γ. Induction of A(α)-specific mRNA was sensitive to the protein synthesis inhibitor cycloheximide. Simultaneous treatment of macrophage cultures with rIFN-γ and IFN-α/β or the glucocorticoid dexamethasone (DEX) resulted in a significant decrease in steady-state, A(α)-specific mRNA levels compared with treatment with rIFN-γ alone. This analysis suggests that both the induction of Ia expression by rIFN-γ, and the antagonism of rIFN-γ-induced Ia gene expression by IFN-α/β and DEX, are regulated by cognate changes in Ia mRNA.

Original languageEnglish (US)
Pages (from-to)244-249
Number of pages6
JournalJournal of Immunology
Volume139
Issue number1
StatePublished - Aug 21 1987
Externally publishedYes

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Histocompatibility Antigens Class II
Dexamethasone
Down-Regulation
Macrophages
Messenger RNA
Glucocorticoids
Protein Synthesis Inhibitors
Peritoneal Macrophages
Exudates and Transudates
Cycloheximide
Gene Expression

ASJC Scopus subject areas

  • Immunology

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Induction of macrophage Ia antigen expression by rIFN-γ and down-regulation by IFN-α/β and dexamethasone are mediated by changes in steady-state levels of Ia mRNA. / Fertsch, Diana; Schoenberg, D. R.; Germain, R. N.; Tou, J. Y.; Vogel, S. N.

In: Journal of Immunology, Vol. 139, No. 1, 21.08.1987, p. 244-249.

Research output: Contribution to journalArticle

Fertsch, Diana ; Schoenberg, D. R. ; Germain, R. N. ; Tou, J. Y. ; Vogel, S. N. / Induction of macrophage Ia antigen expression by rIFN-γ and down-regulation by IFN-α/β and dexamethasone are mediated by changes in steady-state levels of Ia mRNA. In: Journal of Immunology. 1987 ; Vol. 139, No. 1. pp. 244-249.
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