Induction of human immunodeficiency virus type 1 expression in chronically infected cells is associated primarily with a shift in RNA snlicins patterns

Nelson L. Michael, Paul Morrow, Joseph Mosca, Mary Anne Vahey, Donald S. Burke, Robert R. Redfield

Research output: Contribution to journalArticle

Abstract

We have analyzed the kinetics of human immunodeficiency virus type l (HIV-1) RNA induction in chronically infected T cells and promonocytes. A substantial amount of spliced mRNAs and assembled virions was found in resting cells. Induction increased the steady-state level of total HIV-1 RNA by 4-fold but increased the level of unsplked transcripts by 25-fold. This increase in unspliced RNA was reflected in the amount of virus seen by electron microscopy. These data suggest a mechanism for the induction of HIV-1 RNA in chronically infected cells involving a shift in splicing greatly favoring the stability of unspliced viral RNA with only a modest increase in total viral RNA. Analysis of the relative abundance of transcript classes is critical to the measurement of HIV-1 viral replication kinetics.

Original languageEnglish (US)
Pages (from-to)1291-1303
Number of pages13
JournalJournal of virology
Volume65
Issue number3
StatePublished - Dec 1 1991

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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    Michael, N. L., Morrow, P., Mosca, J., Vahey, M. A., Burke, D. S., & Redfield, R. R. (1991). Induction of human immunodeficiency virus type 1 expression in chronically infected cells is associated primarily with a shift in RNA snlicins patterns. Journal of virology, 65(3), 1291-1303.