Induction of heat-shock gene expression in postischemic pig liver depends on superoxide generation

Luke O. Schoeniger, Kenneth A. Andreoni, Gregory R. Ott, Terence H Risby, Gregory B. Bulkley, Robert Udelsman, James F. Burdick, Timothy G. Buchman

Research output: Contribution to journalArticle

Abstract

Background/Aims: Both hemorrhagic and cardiogenic shock are associated with hepatic shock gene expression at resuscitation. This study investigated the potential role of intravascular superoxide anion as a proximal trigger of heat shock protein gene expression. Methods: Preanesthetized pigs were subjected to 120 m of total warm hepatic ischemia. The survival model consisted of warm, total hepatic ischemia and reperfusion (with active portal-systemic bypass) followed by reperfusion and survival for 3 days. Serial hepatic biopsy samples were evaluated for the expression of heat shock protein 72 (HSP-72) messenger RNA (mRNA) by Northern and Western analysis and by in situ RNA hybridization. The possible role of intravascular O2- as a mediator of heat shock response was evaluated by its specific inhibition by the intravenous infusion of recombinant human superoxide dismutase (SOD). Results: Ischemia for 120 minutes followed by 60 minutes of reperfusion caused accumulation of HSP-72 mRNA. Transcripts were localized to hepatocytes. HSP-72 mRNA was detected neither following ischemia alone nor when SOD was infused for 15 minutes at reperfusion. Three days later, transcripts were not detectable, but HSP-72 protein accumulated irrespective of SOD administration. Conclusions: Warm hepatic ischemia induces the hepatocyte expression of HSP-72 at reperfusion by a mechanism that is dependent upon the superoxide anion, probably generated intravascularly. However, the transient dismutation of superoxide is insufficient to suppress subsequent accumulation of HSP-72.

Original languageEnglish (US)
Pages (from-to)177-184
Number of pages8
JournalGastroenterology
Volume106
Issue number1
StatePublished - 1994

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HSP72 Heat-Shock Proteins
Superoxides
Shock
Swine
Hot Temperature
Reperfusion
Gene Expression
Liver
Superoxide Dismutase
Warm Ischemia
Ischemia
Messenger RNA
Hepatocytes
Heat-Shock Response
Survival
Cardiogenic Shock
Hemorrhagic Shock
Heat-Shock Proteins
Intravenous Infusions
Resuscitation

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Schoeniger, L. O., Andreoni, K. A., Ott, G. R., Risby, T. H., Bulkley, G. B., Udelsman, R., ... Buchman, T. G. (1994). Induction of heat-shock gene expression in postischemic pig liver depends on superoxide generation. Gastroenterology, 106(1), 177-184.

Induction of heat-shock gene expression in postischemic pig liver depends on superoxide generation. / Schoeniger, Luke O.; Andreoni, Kenneth A.; Ott, Gregory R.; Risby, Terence H; Bulkley, Gregory B.; Udelsman, Robert; Burdick, James F.; Buchman, Timothy G.

In: Gastroenterology, Vol. 106, No. 1, 1994, p. 177-184.

Research output: Contribution to journalArticle

Schoeniger, LO, Andreoni, KA, Ott, GR, Risby, TH, Bulkley, GB, Udelsman, R, Burdick, JF & Buchman, TG 1994, 'Induction of heat-shock gene expression in postischemic pig liver depends on superoxide generation', Gastroenterology, vol. 106, no. 1, pp. 177-184.
Schoeniger LO, Andreoni KA, Ott GR, Risby TH, Bulkley GB, Udelsman R et al. Induction of heat-shock gene expression in postischemic pig liver depends on superoxide generation. Gastroenterology. 1994;106(1):177-184.
Schoeniger, Luke O. ; Andreoni, Kenneth A. ; Ott, Gregory R. ; Risby, Terence H ; Bulkley, Gregory B. ; Udelsman, Robert ; Burdick, James F. ; Buchman, Timothy G. / Induction of heat-shock gene expression in postischemic pig liver depends on superoxide generation. In: Gastroenterology. 1994 ; Vol. 106, No. 1. pp. 177-184.
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AU - Udelsman, Robert

AU - Burdick, James F.

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