Induction of guinea pig antibody responses in vitro

P. L. Cohen, J. J. Ellner, E. M. Shevach

Research output: Contribution to journalArticle

Abstract

Guinea pig spleen cells cultured together with peritoneal exudate lymphocytes (PEL) were found to generate large numbers of antibody forming cells (AFC) in vitro in response to hapten protein antigens. Neither cell type cultured alone yielded appreciable responses. Strain 13 or F1 (Strain 2 x strain 13) lymphocytes, but not those from strain 2 animals, are able to respond to the genetically controlled antigen, DNP guinea pig albumin (DNP-GPA). Antisera directed against responder (strain 13) parent Ia antigens selectively blocked the generation of AFC by F1 (strain 2 x strain 13) spleen PEL mixtures in response to DNP-GPA. Both allogeneic (strain 2) and syngeneic macrophages functioned equally well in presentation of DNP-GPA to strain 13 lymphocytes.

Original languageEnglish (US)
Pages (from-to)2034-2039
Number of pages6
JournalJournal of Immunology
Volume118
Issue number6
StatePublished - 1977
Externally publishedYes

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Antibody Formation
Guinea Pigs
Lymphocytes
Albumins
Exudates and Transudates
Cultured Cells
Spleen
Antigens
Antibodies
Haptens
Histocompatibility Antigens Class II
Immune Sera
Macrophages
In Vitro Techniques
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Cohen, P. L., Ellner, J. J., & Shevach, E. M. (1977). Induction of guinea pig antibody responses in vitro. Journal of Immunology, 118(6), 2034-2039.

Induction of guinea pig antibody responses in vitro. / Cohen, P. L.; Ellner, J. J.; Shevach, E. M.

In: Journal of Immunology, Vol. 118, No. 6, 1977, p. 2034-2039.

Research output: Contribution to journalArticle

Cohen, PL, Ellner, JJ & Shevach, EM 1977, 'Induction of guinea pig antibody responses in vitro', Journal of Immunology, vol. 118, no. 6, pp. 2034-2039.
Cohen PL, Ellner JJ, Shevach EM. Induction of guinea pig antibody responses in vitro. Journal of Immunology. 1977;118(6):2034-2039.
Cohen, P. L. ; Ellner, J. J. ; Shevach, E. M. / Induction of guinea pig antibody responses in vitro. In: Journal of Immunology. 1977 ; Vol. 118, No. 6. pp. 2034-2039.
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