Induction of experimental allergic encephalomyelitis in the NIH minipig

B. A. Singer, N. J. Tresser, J. A. Frank, H. F. McFarland, W. E. Biddison

Research output: Contribution to journalArticle

Abstract

Experimental allergic encephalomyelitis (EAE) was induced in the NIH minipig to create a large animal model of multiple sclerosis with a well-characterized immune system. Sixteen NIH minipigs were inoculated with minipig spinal cord homogenate (SCH). The clinical course was primarily monophasic, but re-induction was possible. CNS and blood lymphocytes specifically proliferated to SCH. Flow cytometry of CNS-isolated cells and SCH-stimulated PBMC revealed a shift to CD4+ CD8+ cells. Pathology demonstrated demyelination in the CNS white matter with perivascular mononuclear cell infiltrates of CD3+CD4+CD45+ lymphocytes with a subset CD8+. Pathology and in vitro SCH responses implicate a central role of CD4+ lymphocytes in swine EAE. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)7-19
Number of pages13
JournalJournal of Neuroimmunology
Volume105
Issue number1
DOIs
StatePublished - Jun 1 2000
Externally publishedYes

Keywords

  • Demyelination
  • Experimental allergic encephalomyelitis
  • Lymphocyte
  • Magnetic resonance imaging
  • Minipig
  • Multiple sclerosis

ASJC Scopus subject areas

  • Immunology
  • Clinical Neurology
  • Immunology and Allergy
  • Neurology

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  • Cite this

    Singer, B. A., Tresser, N. J., Frank, J. A., McFarland, H. F., & Biddison, W. E. (2000). Induction of experimental allergic encephalomyelitis in the NIH minipig. Journal of Neuroimmunology, 105(1), 7-19. https://doi.org/10.1016/S0165-5728(99)00275-1