Abstract
We treated KU812 cells from different passage levels with compounds which induce differentiation of other leukemic cell lines. Early passages contained small subpopulations of basophil and erythrocyte-like cells, but later passages consisted of more uniform cells with a myelomonocytic phenotype. When cultured with I nM actinomycin D, the cells became nonproliferative, developed pale cytoplasm and segmented nuclei, and lost nonspecific esterase activity. Thus, KU812 cells can be induced to mature to cells with a myelomonocytic phenotype. However, their expression of previously reported basophil characteristics diminishes with continued passage, and may be a capability of only a subpopulation of the cells.
Original language | English (US) |
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Pages (from-to) | 1105-1107,1109-1113 |
Journal | Leukemia Research |
Volume | 11 |
Issue number | 12 |
DOIs | |
State | Published - 1987 |
Externally published | Yes |
Keywords
- KU812
- actinomycin D
- differentiation
- leukemia
ASJC Scopus subject areas
- Hematology
- Oncology
- Cancer Research