Induction of differentiation of the human leukemia cell line, KU812

Steven A. Fischkoff, Kenji Kishi, William R. Benjamin, Randall M. Rossi, Michel C. Hoessly, James A. Hoxie, Cary Seals, Linda Anderson, Teruko Ishizaka, John Hakimi

Research output: Contribution to journalArticlepeer-review

Abstract

We treated KU812 cells from different passage levels with compounds which induce differentiation of other leukemic cell lines. Early passages contained small subpopulations of basophil and erythrocyte-like cells, but later passages consisted of more uniform cells with a myelomonocytic phenotype. When cultured with I nM actinomycin D, the cells became nonproliferative, developed pale cytoplasm and segmented nuclei, and lost nonspecific esterase activity. Thus, KU812 cells can be induced to mature to cells with a myelomonocytic phenotype. However, their expression of previously reported basophil characteristics diminishes with continued passage, and may be a capability of only a subpopulation of the cells.

Original languageEnglish (US)
Pages (from-to)1105-1107,1109-1113
JournalLeukemia Research
Volume11
Issue number12
DOIs
StatePublished - 1987

Keywords

  • KU812
  • actinomycin D
  • differentiation
  • leukemia

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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