Induction of dendritic cell production of type i and type iii interferons by wild-type and vaccine strains of measles virus

Role of defective interfering RNAs

Rupak Shivakoti, Martina Siwek, Debra Hauer, Kimberly L W Schultz, Diane Griffin

Research output: Contribution to journalArticle

Abstract

The innate immune response to viral infection frequently includes induction of type I interferons (IFN), but many viruses have evolved ways to block this response and increase virulence. In vitro studies of IFN production after infection of susceptible cells with measles virus (MeV) have often reported greater IFN synthesis after infection with vaccine than with wild-type strains of MeV. However, the possible presence in laboratory virus stocks of 5' copy-back defective interfering (DI) RNAs that induce IFN independent of the standard virus has frequently confounded interpretation of data from these studies. To further investigate MeV strain-dependent differences in IFN induction and the role of DI RNAs, monocyte-derived dendritic cells (moDCs) were infected with the wild-type Bilthoven strain and the vaccine Edmonston-Zagreb strain with and without DI RNAs. Production of type I IFN, type III IFN, and the interferon-stimulated genes (ISGs) Mx and ISG56 by infected cells was assessed with a flow cytometry-based IFN bioassay, quantitative reverse transcriptase PCR (RT-PCR), and immunoassays. Bilthoven infected moDCs less efficiently than Edmonston-Zagreb. Presence of DI RNAs in vaccine stocks resulted in greater maturation of moDCs, inhibition of virus replication, and induction of higher levels of IFN and ISGs. Production of type I IFN, type III IFN, and ISG mRNA and protein was determined by both the level of infection and the presence of DI RNAs. At the same levels of infection and in the absence of DI RNA, IFN induction was similar between wild-type and vaccine strains of MeV.

Original languageEnglish (US)
Pages (from-to)7816-7827
Number of pages12
JournalJournal of Virology
Volume87
Issue number14
DOIs
StatePublished - Jul 2013

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Measles virus
interferons
dendritic cells
Dendritic Cells
Interferons
Vaccines
RNA
vaccines
Interferon Type I
Monocytes
monocytes
Viruses
Infection
infection
viruses
Virus Activation
Virus Diseases
Virus Replication
Reverse Transcriptase Polymerase Chain Reaction
Immunoassay

ASJC Scopus subject areas

  • Immunology
  • Virology

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Induction of dendritic cell production of type i and type iii interferons by wild-type and vaccine strains of measles virus : Role of defective interfering RNAs. / Shivakoti, Rupak; Siwek, Martina; Hauer, Debra; Schultz, Kimberly L W; Griffin, Diane.

In: Journal of Virology, Vol. 87, No. 14, 07.2013, p. 7816-7827.

Research output: Contribution to journalArticle

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