Abstract
Cyclo-oxygenase-2 (COX-2) is believed to participate in the repair of gastric ulcer. Like humans, pigs frequently develop gastric ulcers and thus represent an attractive animal model in which to study the repair process of naturally occurring gastric ulcers. However, expression of COX in the pig stomach has not been reported. The objectives of this study were to determine whether COX isoenzymes are expressed in porcine gastric ulcers and to characterize the porcine COX-2 cDNA. Normal stomachs (n=5) and those with gastric ulcers (n=35) were studied by immunohistochemistry and immunoblotting analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to isolate the complete porcine COX-2 cDNA. COX-1 staining was present in normal stomach and in ulcerated areas. No COX-2 was detected in normal stomach, but COX-2 was strongly expressed in the ulcerated area in 28/35 (80%) gastric ulcers (p
| Original language | English (US) |
|---|---|
| Pages (from-to) | 923-933 |
| Number of pages | 11 |
| Journal | Journal of Histochemistry and Cytochemistry |
| Volume | 50 |
| Issue number | 7 |
| State | Published - 2002 |
| Externally published | Yes |
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Keywords
- Cyclo-oxygenase-2
- Gastric ulcers
- Pigs
- Stomach
ASJC Scopus subject areas
- Cell Biology
- Anatomy
Cite this
Induction of cyclo-oxygenase-2 expression in naturally occurring gastric ulcers. / Lajoie, Stephane; Sirois, Jean; Doré, Monique.
In: Journal of Histochemistry and Cytochemistry, Vol. 50, No. 7, 2002, p. 923-933.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Induction of cyclo-oxygenase-2 expression in naturally occurring gastric ulcers
AU - Lajoie, Stephane
AU - Sirois, Jean
AU - Doré, Monique
PY - 2002
Y1 - 2002
N2 - Cyclo-oxygenase-2 (COX-2) is believed to participate in the repair of gastric ulcer. Like humans, pigs frequently develop gastric ulcers and thus represent an attractive animal model in which to study the repair process of naturally occurring gastric ulcers. However, expression of COX in the pig stomach has not been reported. The objectives of this study were to determine whether COX isoenzymes are expressed in porcine gastric ulcers and to characterize the porcine COX-2 cDNA. Normal stomachs (n=5) and those with gastric ulcers (n=35) were studied by immunohistochemistry and immunoblotting analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to isolate the complete porcine COX-2 cDNA. COX-1 staining was present in normal stomach and in ulcerated areas. No COX-2 was detected in normal stomach, but COX-2 was strongly expressed in the ulcerated area in 28/35 (80%) gastric ulcers (p
AB - Cyclo-oxygenase-2 (COX-2) is believed to participate in the repair of gastric ulcer. Like humans, pigs frequently develop gastric ulcers and thus represent an attractive animal model in which to study the repair process of naturally occurring gastric ulcers. However, expression of COX in the pig stomach has not been reported. The objectives of this study were to determine whether COX isoenzymes are expressed in porcine gastric ulcers and to characterize the porcine COX-2 cDNA. Normal stomachs (n=5) and those with gastric ulcers (n=35) were studied by immunohistochemistry and immunoblotting analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to isolate the complete porcine COX-2 cDNA. COX-1 staining was present in normal stomach and in ulcerated areas. No COX-2 was detected in normal stomach, but COX-2 was strongly expressed in the ulcerated area in 28/35 (80%) gastric ulcers (p
KW - Cyclo-oxygenase-2
KW - Gastric ulcers
KW - Pigs
KW - Stomach
UR - http://www.scopus.com/inward/record.url?scp=0036067452&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036067452&partnerID=8YFLogxK
M3 - Article
C2 - 12070271
AN - SCOPUS:0036067452
VL - 50
SP - 923
EP - 933
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 7
ER -