Induction of cyclo-oxygenase-2 expression in naturally occurring gastric ulcers

Stephane Lajoie, Jean Sirois, Monique Doré

Research output: Contribution to journalArticle

Abstract

Cyclo-oxygenase-2 (COX-2) is believed to participate in the repair of gastric ulcer. Like humans, pigs frequently develop gastric ulcers and thus represent an attractive animal model in which to study the repair process of naturally occurring gastric ulcers. However, expression of COX in the pig stomach has not been reported. The objectives of this study were to determine whether COX isoenzymes are expressed in porcine gastric ulcers and to characterize the porcine COX-2 cDNA. Normal stomachs (n=5) and those with gastric ulcers (n=35) were studied by immunohistochemistry and immunoblotting analysis. Reverse transcription-polymerase chain reaction (RT-PCR) was used to isolate the complete porcine COX-2 cDNA. COX-1 staining was present in normal stomach and in ulcerated areas. No COX-2 was detected in normal stomach, but COX-2 was strongly expressed in the ulcerated area in 28/35 (80%) gastric ulcers (p

Original languageEnglish (US)
Pages (from-to)923-933
Number of pages11
JournalJournal of Histochemistry and Cytochemistry
Volume50
Issue number7
StatePublished - 2002
Externally publishedYes

Fingerprint

Stomach Ulcer
Prostaglandin-Endoperoxide Synthases
Swine
Stomach
Complementary DNA
Immunoblotting
Isoenzymes
Reverse Transcription
Animal Models
Immunohistochemistry
Staining and Labeling
Polymerase Chain Reaction

Keywords

  • Cyclo-oxygenase-2
  • Gastric ulcers
  • Pigs
  • Stomach

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Induction of cyclo-oxygenase-2 expression in naturally occurring gastric ulcers. / Lajoie, Stephane; Sirois, Jean; Doré, Monique.

In: Journal of Histochemistry and Cytochemistry, Vol. 50, No. 7, 2002, p. 923-933.

Research output: Contribution to journalArticle

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