Induction of calcium flux across the rat mast cell membrane by bridging IgE receptors

T. Ishizaka, J. C. Foreman, A. R. Sterk, K. Ishizaka

Research output: Contribution to journalArticle

Abstract

Rabbit antibody against IgE receptors or its F(ab')2 fragments induced an increase of 45Ca uptake into normal mast cells, and this process was accompanied by histamine release. Evidence was obtained that a substantial portion of 45Ca uptake was due to movement of calcium across the cell membrane. Neither 45Ca uptake nor histamine release was induced by Fab' monomer fragments of the antireceptor antibody. However, bridging of receptor-bound Fab' fragments by anti-rabbit IgG or bridging of receptor-bound IgE by anti-IgE induced an increase in 45Ca uptake. The results collectively indicate that crosslinking IgE receptors increases the membrane permeability of the mast cells toward calcium.

Original languageEnglish (US)
Pages (from-to)5858-5862
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume76
Issue number11
StatePublished - 1979

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IgE Receptors
Mast Cells
Immunoglobulin Fab Fragments
Histamine Release
Cell Membrane
Calcium
Rabbits
Antibodies
Permeability
Membranes

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Induction of calcium flux across the rat mast cell membrane by bridging IgE receptors. / Ishizaka, T.; Foreman, J. C.; Sterk, A. R.; Ishizaka, K.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 76, No. 11, 1979, p. 5858-5862.

Research output: Contribution to journalArticle

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