Induction of c-foS mRNA by lead in PC12 cells

K. Kim, M. Annadata, G. W. Goldstein, J. P. Bressler

Research output: Contribution to journalArticlepeer-review

Abstract

Addition of lead acetate to PC12 pheochromocytoma cells elicits induction of c-fos, an immediate early response gene. Induction of c-fos was concentration- and time-dependent: the lowest concentration of lead acetate tested that induced c-fos was 10 μM; induction was observed after a 30 min incubation and remained high after 90 min. Treatment with lead acetate and cycloheximide superinduced c-fos mRNA. Actinomycin D, an inhibitor of mRNA transcription, decreased the level of c-fos mRNA induced by lead acetate by almost 80%. Cadmium chloride and zinc chloride did not induce c-fos mRNA. Since the c-fos gene encodes a transcription factor, Pb2+ has the potential to deregulate the expression of other genes.

Original languageEnglish (US)
Pages (from-to)175-182
Number of pages8
JournalInternational Journal of Developmental Neuroscience
Volume15
Issue number2
DOIs
StatePublished - Apr 1997

Keywords

  • Genes
  • PC12
  • Pb
  • fos

ASJC Scopus subject areas

  • Developmental Neuroscience
  • Developmental Biology

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