Induction of bone marrow stromal cells into GABAergic neuronal phenotype using creatine as inducer

Payam Mohammadgharibani, Taki Tiraihi, Seyed Alireza Mesbah-Namin, Jalil Arabkheradmand, Hadi Kazemi

Research output: Contribution to journalArticle

Abstract

Purpose: Deficits involving GABAergic neurons have been reported in aging, central nervous trauma and neurodegenerative disorders; bone marrow stromal cells (BMSCs) have been proposed as a feasible source of donor cells in replacement cell therapy. In this study, the effects of creatine on transdifferentiating BMSCs into GABAergic-like neurons were evaluated in vitro. Methods: The BMSCs were isolated from adult rats, preinduced by β-mercaptoethanol (BME) and retinoic acid (RA), and then induced by creatine into GABAergic-like neurons. The cells were characterized using different differentiation markers. The functionality of the differentiated cells was evaluated using FM1-43. Results: The isolated cells expressed Oct-4 and were immunoreactive to fibronectin and CD44. The highest percentages of cells immunoreactive to nestin and neurofilament-68 were found at the second day of preinduction. At the induction stage, there were increases in the number of cells immunoreactive to neurofilament-200, MAP-2, synapsin I, synaptophysin, and NeuN. The percentages of the immunoreactive cells to GABAergic neuron markers increased. The optimal induction dose was 5 mM. The dose of 10 mM showed a decline in the expression of most of these markers. The functionality test indicated that the synaptic vesicles were released upon stimulation. Conclusion: Creatine can induce the differentiation of BMSCs to the GABAergic neuronal phenotype within one week.

Original languageEnglish (US)
Pages (from-to)511-525
Number of pages15
JournalRestorative Neurology and Neuroscience
Volume30
Issue number6
DOIs
StatePublished - Dec 1 2012
Externally publishedYes

Fingerprint

Creatine
Mesenchymal Stromal Cells
GABAergic Neurons
Phenotype
Synapsins
Nestin
Synaptophysin
Intermediate Filaments
Mercaptoethanol
Synaptic Vesicles
Differentiation Antigens
Cell- and Tissue-Based Therapy
Tretinoin
Fibronectins
Neurodegenerative Diseases
Cell Count
Wounds and Injuries

Keywords

  • BMSCs
  • creatine
  • GABAergic-like neurons
  • induction

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience
  • Clinical Neurology

Cite this

Induction of bone marrow stromal cells into GABAergic neuronal phenotype using creatine as inducer. / Mohammadgharibani, Payam; Tiraihi, Taki; Mesbah-Namin, Seyed Alireza; Arabkheradmand, Jalil; Kazemi, Hadi.

In: Restorative Neurology and Neuroscience, Vol. 30, No. 6, 01.12.2012, p. 511-525.

Research output: Contribution to journalArticle

Mohammadgharibani, Payam ; Tiraihi, Taki ; Mesbah-Namin, Seyed Alireza ; Arabkheradmand, Jalil ; Kazemi, Hadi. / Induction of bone marrow stromal cells into GABAergic neuronal phenotype using creatine as inducer. In: Restorative Neurology and Neuroscience. 2012 ; Vol. 30, No. 6. pp. 511-525.
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