Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line

Monica Boveri, Vincent Berezowski, Anna Price, Stephanie Slupek, Anne Marie Lenfant, Christelle Benaud, Thomas Hartung, Romeo Cecchelli, Pilar Prieto, Marie Pierre Dehouck

Research output: Contribution to journalArticle

Abstract

The communication between glial cells and brain capillary endothelial cells is crucial for a well-differentiated blood-brain barrier (BBB). It has been suggested that in vitro primary glial cells (GCs) be replaced by the glial C6 cell line to standardise the model further. This study compares directly the structural and functional differentiation of bovine brain capillary endothelial cells (BBCECs) induced by co-culture with rat primary GCs or C6 cells, for the first time. Transendothelial electrical resistance (TEER) measurements showed that under no condition were C6 cells able to reproduce TEER values as high as in the presence of GCs. At the same time, permeability of the BBCECs to both radioactive sucrose and FITC-inulin was 2.5-fold higher when cells were co-cultured with C6 than with GCs. Furthermore, immunocytochemistry studies showed different cell morphology and less developed tight junction pattern of BBCECs co-cultured with C6 toward GCs. Additionally, studies on P-glycoprotein (P-gp) showed much lower P-gp presence and activity in BBCECs co-cultured with C6 than GCs. Both VEGF mRNA expression and protein content were dramatically increased when compared with GCs, suggesting that VEGF could be one of the factors responsible for higher permeability of BBB. Our results clearly indicate that, in the presence of the glial C6 cell line, BBCECs did not differentiate as well as in the coculture with primary GCs at both structural and functional levels.

Original languageEnglish (US)
Pages (from-to)187-198
Number of pages12
JournalGLIA
Volume51
Issue number3
DOIs
StatePublished - Aug 15 2005
Externally publishedYes

Fingerprint

Blood-Brain Barrier
Neuroglia
Endothelial Cells
Cell Line
Brain
P-Glycoprotein
Coculture Techniques
Electric Impedance
Vascular Endothelial Growth Factor A
Permeability
Tight Junctions
Sucrose
Immunohistochemistry
Communication
Messenger RNA

Keywords

  • Blood-brain barrier
  • C6
  • Endothelial cells
  • In vitro model
  • Primary glial cells

ASJC Scopus subject areas

  • Immunology

Cite this

Boveri, M., Berezowski, V., Price, A., Slupek, S., Lenfant, A. M., Benaud, C., ... Dehouck, M. P. (2005). Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line. GLIA, 51(3), 187-198. https://doi.org/10.1002/glia.20189

Induction of blood-brain barrier properties in cultured brain capillary endothelial cells : Comparison between primary glial cells and C6 cell line. / Boveri, Monica; Berezowski, Vincent; Price, Anna; Slupek, Stephanie; Lenfant, Anne Marie; Benaud, Christelle; Hartung, Thomas; Cecchelli, Romeo; Prieto, Pilar; Dehouck, Marie Pierre.

In: GLIA, Vol. 51, No. 3, 15.08.2005, p. 187-198.

Research output: Contribution to journalArticle

Boveri, M, Berezowski, V, Price, A, Slupek, S, Lenfant, AM, Benaud, C, Hartung, T, Cecchelli, R, Prieto, P & Dehouck, MP 2005, 'Induction of blood-brain barrier properties in cultured brain capillary endothelial cells: Comparison between primary glial cells and C6 cell line', GLIA, vol. 51, no. 3, pp. 187-198. https://doi.org/10.1002/glia.20189
Boveri, Monica ; Berezowski, Vincent ; Price, Anna ; Slupek, Stephanie ; Lenfant, Anne Marie ; Benaud, Christelle ; Hartung, Thomas ; Cecchelli, Romeo ; Prieto, Pilar ; Dehouck, Marie Pierre. / Induction of blood-brain barrier properties in cultured brain capillary endothelial cells : Comparison between primary glial cells and C6 cell line. In: GLIA. 2005 ; Vol. 51, No. 3. pp. 187-198.
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