Induction of angiogenic chemokine CCL2 by human herpesvirus 8 chemokine receptor

Research output: Contribution to journalArticle

Abstract

Human herpesvirus 8 (HHV-8) is associated with Kaposi's sarcoma (KS), an endothelial cell lesion believed to be initiated and driven primarily by cytokine dysregulation. Among the viral proteins suspected as contributing to viral pathogenesis is the lytically expressed viral G protein-coupled receptor (vGPCR), which can induce various cellular cytokines. CC ligand-2 (CCL2/MCP-1) is a vGPCR-regulated angiogenic chemokine found at elevated levels in KS lesions and induced by HHV-8 infection of endothelial cells. Here we show that vGPCR induces CCL2 in endothelial cells via activation of C/EBPβ and that vGPCR and C/EBPβ are critical components of CCL2 induction by HHV-8 infection of endothelial cultures. To our knowledge, this is the first report of vGPCR-mediated cytokine induction, and its characterization, in the context of virus infection. Our results identify a mechanism by which vGPCR can contribute, in a host cell shutoff-independent manner, to viral pathogenesis.

Original languageEnglish (US)
Pages (from-to)369-378
Number of pages10
JournalVirology
Volume397
Issue number2
DOIs
StatePublished - Feb 20 2010

Keywords

  • Angiogenesis
  • C/EBPβ
  • CCL2
  • HHV-8
  • MCP-1
  • ORF74
  • Transcription
  • vGPCR

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Induction of angiogenic chemokine CCL2 by human herpesvirus 8 chemokine receptor'. Together they form a unique fingerprint.

  • Cite this