Induction of alpha interferon by human immunodeficiency virus type 1 in human monocyte-macrophage cultures

J. Szebeni; C. Dieffenbach; S. M. Wahl; C. N. Venkateshan; A. Yeh; M. Popovic; S. Gartner; L. M. Wahl; M. Peterfy; R. M. Friedman; J. N. Weinstein

Induction of alpha interferon by human immunodeficiency virus type 1 in human monocyte-macrophage cultures

J. Szebeni, C. Dieffenbach, S. M. Wahl, C. N. Venkateshan, A. Yeh, M. Popovic, S. Gartner, L. M. Wahl, M. Peterfy, R. M. Friedman, J. N. Weinstein

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

The induction of interferon (IFN) by human immunodeficiency virus type 1 (HIV-1) in primary, nonstimulated monocyte-macrophage cultures was studied. HIV-1 infection, as confirmed by p24 antigen levels in the cell supernatant, led to the production of alpha interferon (IFN-α) over 7 to 21 days following infection. In two of seven experiments, the IFN detected was acid labile. Coupled reverse transcription-polymerase chain reaction analysis confirmed the induction of IFN-α mRNA in cells of HIV-1-infected cultures.

Original language	English (US)
Pages (from-to)	6362-6364
Number of pages	3
Journal	Journal of Virology
Volume	65
Issue number	11
State	Published - Nov 1991
Externally published	Yes

ASJC Scopus subject areas

Immunology

Cite this

@article{f1e4b00066ab454d99b5e5fca0d34e2f,

title = "Induction of alpha interferon by human immunodeficiency virus type 1 in human monocyte-macrophage cultures",

abstract = "The induction of interferon (IFN) by human immunodeficiency virus type 1 (HIV-1) in primary, nonstimulated monocyte-macrophage cultures was studied. HIV-1 infection, as confirmed by p24 antigen levels in the cell supernatant, led to the production of alpha interferon (IFN-α) over 7 to 21 days following infection. In two of seven experiments, the IFN detected was acid labile. Coupled reverse transcription-polymerase chain reaction analysis confirmed the induction of IFN-α mRNA in cells of HIV-1-infected cultures.",

author = "J. Szebeni and C. Dieffenbach and Wahl, {S. M.} and Venkateshan, {C. N.} and A. Yeh and M. Popovic and S. Gartner and Wahl, {L. M.} and M. Peterfy and Friedman, {R. M.} and Weinstein, {J. N.}",

year = "1991",

month = nov,

language = "English (US)",

volume = "65",

pages = "6362--6364",

journal = "Journal of Virology",

issn = "0022-538X",

publisher = "American Society for Microbiology",

number = "11",

}

TY - JOUR

T1 - Induction of alpha interferon by human immunodeficiency virus type 1 in human monocyte-macrophage cultures

AU - Szebeni, J.

AU - Dieffenbach, C.

AU - Wahl, S. M.

AU - Venkateshan, C. N.

AU - Yeh, A.

AU - Popovic, M.

AU - Gartner, S.

AU - Wahl, L. M.

AU - Peterfy, M.

AU - Friedman, R. M.

AU - Weinstein, J. N.

PY - 1991/11

Y1 - 1991/11

N2 - The induction of interferon (IFN) by human immunodeficiency virus type 1 (HIV-1) in primary, nonstimulated monocyte-macrophage cultures was studied. HIV-1 infection, as confirmed by p24 antigen levels in the cell supernatant, led to the production of alpha interferon (IFN-α) over 7 to 21 days following infection. In two of seven experiments, the IFN detected was acid labile. Coupled reverse transcription-polymerase chain reaction analysis confirmed the induction of IFN-α mRNA in cells of HIV-1-infected cultures.

AB - The induction of interferon (IFN) by human immunodeficiency virus type 1 (HIV-1) in primary, nonstimulated monocyte-macrophage cultures was studied. HIV-1 infection, as confirmed by p24 antigen levels in the cell supernatant, led to the production of alpha interferon (IFN-α) over 7 to 21 days following infection. In two of seven experiments, the IFN detected was acid labile. Coupled reverse transcription-polymerase chain reaction analysis confirmed the induction of IFN-α mRNA in cells of HIV-1-infected cultures.

UR - http://www.scopus.com/inward/record.url?scp=0026044408&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026044408&partnerID=8YFLogxK

M3 - Article

C2 - 1920639

AN - SCOPUS:0026044408

SN - 0022-538X

VL - 65

SP - 6362

EP - 6364

JO - Journal of Virology

JF - Journal of Virology

IS - 11

ER -

Induction of alpha interferon by human immunodeficiency virus type 1 in human monocyte-macrophage cultures

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this