Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape

Ann Sodja, Hisashi Fujioka, Francisco J A Lemos, Marilyn Donnelly-Doman, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticle

Abstract

Ingestion of a blood meal by the female mosquito Anopheles gambiae (L., Diptera: Culicidae), results in a dramatic distention of the midgut epithelium. Here, we report that these events correlate with a transient increase of actin mRNA and protein abundance. The newly synthesized actin may provide a pool of actin protein needed to remodel epithelial cell cytoarchitecture. We also document changes in midgut epithelial cell morphology. Upon blood ingestion, the columnar cells flatten accompanied by the loss of microvilli on the lumenal side and the unfolding of the labyrinth on the basal side. These changes correlate with the large increase of epithelial surface area needed to accommodate the blood meal. Actin gene expression, actin synthesis and cell morphology all return to the pre-feeding state by 24 h after blood intake.

Original languageEnglish (US)
Pages (from-to)833-839
Number of pages7
JournalJournal of Insect Physiology
Volume53
Issue number8
DOIs
StatePublished - Aug 2007

Fingerprint

Cell Shape
Culicidae
midgut
actin
Actins
Eating
ingestion
Gene Expression
gene expression
blood
blood meal
cells
Meals
epithelial cells
Epithelial Cells
Anopheles gambiae
Inner Ear
Microvilli
microvilli
Diptera

Keywords

  • Actin
  • Anopheles gambiae
  • Blood feeding
  • Microvilli
  • Midgut

ASJC Scopus subject areas

  • Insect Science
  • Physiology

Cite this

Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape. / Sodja, Ann; Fujioka, Hisashi; Lemos, Francisco J A; Donnelly-Doman, Marilyn; Jacobs-Lorena, Marcelo.

In: Journal of Insect Physiology, Vol. 53, No. 8, 08.2007, p. 833-839.

Research output: Contribution to journalArticle

Sodja, Ann ; Fujioka, Hisashi ; Lemos, Francisco J A ; Donnelly-Doman, Marilyn ; Jacobs-Lorena, Marcelo. / Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape. In: Journal of Insect Physiology. 2007 ; Vol. 53, No. 8. pp. 833-839.
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