Induction of actin gene expression in the mosquito midgut by blood ingestion correlates with striking changes of cell shape

Ann Sodja, Hisashi Fujioka, Francisco J.A. Lemos, Marilyn Donnelly-Doman, Marcelo Jacobs-Lorena

Research output: Contribution to journalArticle

Abstract

Ingestion of a blood meal by the female mosquito Anopheles gambiae (L., Diptera: Culicidae), results in a dramatic distention of the midgut epithelium. Here, we report that these events correlate with a transient increase of actin mRNA and protein abundance. The newly synthesized actin may provide a pool of actin protein needed to remodel epithelial cell cytoarchitecture. We also document changes in midgut epithelial cell morphology. Upon blood ingestion, the columnar cells flatten accompanied by the loss of microvilli on the lumenal side and the unfolding of the labyrinth on the basal side. These changes correlate with the large increase of epithelial surface area needed to accommodate the blood meal. Actin gene expression, actin synthesis and cell morphology all return to the pre-feeding state by 24 h after blood intake.

Original languageEnglish (US)
Pages (from-to)833-839
Number of pages7
JournalJournal of Insect Physiology
Volume53
Issue number8
DOIs
StatePublished - Aug 1 2007

Keywords

  • Actin
  • Anopheles gambiae
  • Blood feeding
  • Microvilli
  • Midgut

ASJC Scopus subject areas

  • Physiology
  • Insect Science

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