Induction of a type 1 immune response to a recombinant antigen from Mycobacterium tuberculosis expressed in Mycobacterium vaccae

Christiane Abou-Zeid, Marie Pierre Gares, Jacqueline Inwald, Riny Janssen, Ying Zhang, Douglas B. Young, Charlotte Hetzel, Jonathan R. Lamb, Susan L. Baldwin, Ian M. Orme, Vladimir Yeremeev, Boris V. Nikonenko, Alexander S. Apt

Research output: Contribution to journalArticle

Abstract

A 19-kDa lipoprotein from Mycobacterium tuberculosis was expressed as a recombinant antigen in the nonpathogenic mycobacterial host strain M. vaccae. Immunization of mice with the recombinant M. vaccae resulted in induction of a strong type 1 immune response to the 19-kDa antigen, characterized by immunoglobulin G2a (IgG2a) antibodies and gamma interferon (IFN-γ) production by splenocytes. Immunization with the same antigen in incomplete Freund's adjuvant induced a strong IgG1 response with only low levels of IFN-γ. Subsequent intravenous and aerosol challenges of immunized mice with virulent M. tuberculosis demonstrated no evidence of protection associated with the response to the 19-kDa antigen; in fact, the presence of the recombinant 19-kDa antigen abrogated the limited protection conferred by M. vaccae (vector control). The recombinant M. vaccae system is a convenient approach to induction of type 1 responses to M. tuberculosis antigens. However, the unexpected reduction in protective efficacy of M. vaccae expressing the 19-kDa antigen highlights the complexity of testing recombinant subunit vaccines and the need for a better understanding of the immune mechanisms required for effective vaccination against tuberculosis.

Original languageEnglish (US)
Pages (from-to)1856-1862
Number of pages7
JournalInfection and immunity
Volume65
Issue number5
StatePublished - May 17 1997

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Induction of a type 1 immune response to a recombinant antigen from Mycobacterium tuberculosis expressed in Mycobacterium vaccae'. Together they form a unique fingerprint.

  • Cite this

    Abou-Zeid, C., Gares, M. P., Inwald, J., Janssen, R., Zhang, Y., Young, D. B., Hetzel, C., Lamb, J. R., Baldwin, S. L., Orme, I. M., Yeremeev, V., Nikonenko, B. V., & Apt, A. S. (1997). Induction of a type 1 immune response to a recombinant antigen from Mycobacterium tuberculosis expressed in Mycobacterium vaccae. Infection and immunity, 65(5), 1856-1862.