Induction in a Murine Tumor of Immunogenic Tumor Variants by Transfection with a Foreign Gene

Eric R. Fearon, Toshiyuki Itaya, Barbara Hunt, Bert Vogelstein, Philip Frost

Research output: Contribution to journalArticle

Abstract

Transfection of the undifferentiated marine colon carcinoma line CT-26 with the gene coding for the hemagglutination antigen (HA) of influenza virus resulted in the generation of highly immunogenic tumor cells. CT-26 cells transfected with HA not only failed to grow in syngeneic mice but also protected normal animals against a challenge with otherwise lethal doses of parental nontransfected cells. The immunogenicity of HA-transfected cells appeared to correlate with surface HA expression in that tumorigenic clones of HA-transfected CT-26 cells expressed little HA, while immunogenic clones were high expressers of HA. Irradiation of immunogenic HA clones did not abrogate their immunogenicity. These observations demonstrate that immune recognition of a poorly immunogenic tumor can be produced by immunization with tumor cells expressing a defined, foreign cell surface antigen.

Original languageEnglish (US)
Pages (from-to)2975-2980
Number of pages6
JournalCancer Research
Volume48
Issue number11
StatePublished - Jan 1 1988

    Fingerprint

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this